Related Experiment Video
Updated: Jan 10, 2026

A Novel Method for the Culture and Polarized Stimulation of Human Intestinal Mucosa Explants
Published on: May 1, 2013
Immunostimulatory Activity of Solubilized Peptidoglycan Derivatives Prepared From Lactic Acid Bacteria
Sho Noguchi1, Momoko Nakayama2, Masahiro Eguchi2
1Graduate School of Engineering, Kanto Gakuin University, 1-50-1 Mutsuura-higashi Kanazawa-ku Yokohama, Kanagawa, Japan.
Abstract:
To develop vaccine adjuvants from bacterial peptidoglycan (PG) the immunostimulatory activity of lysozyme-solubilized PG derived from Levilactobacillus brevis and Lactiplantibacillus plantarum was investigated. Solubilized PG from both bacteria induced IL-8 in THP-1 cells, and periodate oxidation of L. plantarum PG reduced the activity, suggesting that muramyl dipeptide was partially destroyed. Periodate-oxidized L. plantarum PG showed reduced IL-8 inducing activity in NOD2-expressing cells, while it remained in NOD1-expressing cells, suggesting that γ-d-glutamyl-meso-diaminopimelic acid structure was maintained. All PG preparations stimulated RAW264.7 cells to proliferate, suggesting that they could be potent candidates for vaccine adjuvants.
Related Concept Videos
Peptidoglycan Synthesis
Formation of Lipopolysaccharides
Microorganisms in Medicine and Therapeutics

