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Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

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Published on: January 3, 2025

A long journey towards genome editing technologies in plants: a technical and critical review of genome editing

Dylan Gallo¹, Anne-Cécile Meunier¹, Christophe Périn¹

¹UMR AGAP Institut, CIRAD, INRAE, Institut Agro, University Montpellier, Montpellier, France.

Frontiers in Genome Editing

|November 27, 2025

Abstract:

Advancements in genome editing technologies, notably CRISPR/Cas9, base editing (BE), and prime editing (PE), have revolutionized plant biotechnology, offering unprecedented precision in crop improvement to address the ongoing global warming challenge. This review provides a critical analysis of recent developments in SpCas9-based editing tools, emphasizing enhancements in editing efficiency and specificity and follow the chronological development of editing tools. We explore methodological innovations, including dual pegRNA strategies and site-specific integrases, that have expanded the potential of PE for precise gene insertions. By integrating insights into DNA repair mechanisms and leveraging SpCas9 enhancements, we outline future directions for the application of genome editing in plant breeding.

Keywords:

SpCas9 genome editing knock-in plant prime editing

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