Related Experiment Video
Updated: Jan 10, 2026
05:33
High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds Using HiBiT CRISPR Cell Lines
Published on: November 9, 2020
10.9K
Targeted protein degradation in the transmembrane and extracellular space
Rongfeng Zhu1, Heng Zhang1, Peng R Chen1,2,3,4
1Institute of Chemical Biology, Shenzhen Bay Laboratory, Shenzhen, China.
Science (New York, N.Y.)
|November 27, 2025
Summary
Targeted protein degradation (TPD) is expanding to eliminate extracellular and transmembrane proteins, offering new therapeutic avenues. This review covers membrane-targeted protein degradation (meTPD) technologies, applications, and future potential.
Area of Science:
- Biochemistry
- Molecular Biology
- Drug Discovery
Background:
- Transmembrane and extracellular proteins are vital for cellular functions and disease progression.
- Current targeted protein degradation (TPD) primarily focuses on intracellular targets.
- Expanding TPD to extracellular and transmembrane proteins is a significant advancement.
Purpose of the Study:
- To review targeted protein degradation in the transmembrane and extracellular space (meTPD).
- To discuss platform technologies, features, applications, and limitations of meTPD.
- To highlight conceptual innovations and potential value of meTPD for research and therapeutics.
Main Methods:
- Literature review of emerging meTPD strategies.
- Analysis of platform technologies for targeting extracellular and transmembrane proteins.
- Discussion of current applications and limitations in biological research and therapeutic interventions.
Main Results:
- meTPD strategies are rapidly expanding the scope of targeted protein degradation.
- Various platform technologies are being developed to target proteins outside the cell.
- meTPD holds significant potential for biological research and developing novel therapeutics.
Conclusions:
- meTPD represents a powerful expansion of targeted protein degradation technology.
- Innovations in meTPD are crucial for addressing diseases involving extracellular and transmembrane proteins.
- Further development of meTPD holds promise for future biological research and therapeutic interventions.
Related Concept Videos
02:18The Proteasome
10.0K
Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
10.0K
01:13The Proteasome
1.6K
Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
1.6K
02:18The Proteasome
4.4K
4.4K
02:58Regulated Protein Degradation
8.7K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
8.7K
02:58Regulated Protein Degradation
3.0K
3.0K
01:19Translocation of Proteins into the Mitochondria
11.9K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
11.9K