Selective alkaloid extraction from functional foods via tailored deep eutectic solvent-alkaline electrolyzed water...

Area of Science:

• Phytochemistry
• Green Chemistry
• Biotechnology

Background:

• Nelumbinis Plumula is a valuable medicinal and edible plant with significant pharmaceutical potential.
• Bioactive compounds, particularly alkaloids, are key to its therapeutic properties.
• Efficient and sustainable extraction methods are needed to harness these compounds.

Purpose of the Study:

• To develop a novel, efficient, and sustainable solvent system for alkaloid extraction from Nelumbinis Plumula.
• To optimize the extraction process using a hybrid computational framework.
• To evaluate the green metrics and universality of the developed method.

Main Methods:

• Development of a novel alkaline electrolyzed water and deep eutectic solvent (AEWDES) system.
• Screening of 30 solvents, identifying L-proline-1-methylurea (LP-JJN) as optimal.
• Utilizing a Crested porcupine optimizer-Backpropagation neural networks-Sparrow search algorithm (CPO-BP-SSA) for extraction optimization.
• Assessing extraction efficiency against ultrasound-assisted DES and traditional solvents.

Main Results:

• The developed AEWDES (LP-JJN) demonstrated superior extraction efficiency compared to other methods.
• Yield enhancement is attributed to AEWDES-mediated cell wall disruption and improved intermolecular interactions.
• Green metrics confirmed the sustainability of the AEWDES extraction approach.
• The CPO-BP-SSA framework effectively optimized the alkaloid extraction process.

Conclusions:

• The novel AEWDES extraction system offers a highly efficient and sustainable method for alkaloid recovery from Nelumbinis Plumula.
• This approach provides a universal reference for extracting various alkaloids.
• Future work should focus on advanced techniques for alkaloid compound recovery.