Development of a fluorescence aptasensor based on DNA nanotrain structure for the detection of ampicillin

Ampicillin, a betalactam antibiotic, is used to treat hospital disorders such as gastrointestinal, urogenital, and skin infections. Seizures and hypersensitivity responses are possible adverse effects of this medication. This study aims to qualitatively and quantitatively determine the presence of ampicillin through the use of the fluorescence technique. In this strategy, a DNA nanotrain configuration was developed that consists of a sequence of complementary strands connected to an ampicillin aptamer, which was affixed to a mesoporous silica nanoparticle. The resultant double-stranded segments created an ideal loading zone for the fluorescent compound picogreen, leading to a significant fluorescence emission. Under ideal conditions, the created fluorescent sensing approach demonstrated excellent selectivity for ampicillin, achieving a limit of detection (LOD) of 0.2 nM. The developed aptasensor demonstrated two separate linear intervals, ranging from 0.5 to 10 nM and from 60 to 200 nM. In summary, the suggested approach has the potential to provide a solid basis for the analysis of small molecules, thereby guaranteeing the safety of food and humans by utilizing suitable aptamer sequences.