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Updated: Jan 10, 2026

A Protocol for Explant Cultures of IDH1-mutant Diffuse Low-grade Gliomas
Published on: May 9, 2025
Oncogenic role of IDH1 RNA-binding activity in glioblastoma progression
An Yan1, Wanjun Tang1, Lin Wang1
1State Key Laboratory of Common Mechanism Research for Major Diseases, Department of Biochemistry & Molecular Biology, Medical Primate Research Center, Neuroscience Center, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, 100005, China.
Abstract:
RNA-binding proteins (RBPs) are crucial regulators of gene expression, and their dysfunctions have been implicated in various tumor types. Isocitrate dehydrogenase 1 (IDH1) is a prognostic marker in glioma and has recently been identified as an RBP. This study aimed to investigate the role of IDH1 in glioblastoma (GBM) progression and its regulatory impact on RNA biology. Bioinformatics analysis of The Cancer Genome Atlas (TCGA) data observed that IDH1 expression levels increase with glioma grade and correlate with poor patient survival. Functional assays confirmed that IDH1 acts as an oncogenic factor in GBM cells. Using enhanced crosslinking and immunoprecipitation (eCLIP) and RNA immunoprecipitation (RIP), we identified numerous IDH1-bound transcripts, including p62/SQSTM1 which was validated as a functional target. These findings indicate that IDH1 facilitates glioma progression by binding and suppression of p62.
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