A self-calibrating fluorescence / colorimetric dual-mode sensor based on MIL-101(Fe) for specifical dopamine
Haotian Chen1, Zimo Chen1, Hanbing Yao1
1Department of Pharmaceutical Analysis, School of Pharmacy, China Pharmaceutical University, Nanjing, 211198, China; Key Laboratory of Drug Quality Control and Pharmacovigilance (China Pharmaceutical University), Ministry of Education, Nanjing, 210009, China.
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Dopamine (DA) is an essential biomarker implicated in the diagnosis of neurological diseases. In this work, we designed a dual-enzyme mimetic activity MIL-101(Fe) that catalyzed specific cyclization reaction between 1,3-dihydroxynaphthalene (NR) and dopamine. As the concentration of dopamine increased, the fluorescence emission of NR at 440 nm was markedly quenched, concomitant with the emergence of a new fluorophore exhibiting enhanced emission at 480 nm and increased absorbance at 462 nm. The ratiometric fluorescence/colorimetric dual-mode detection strategy provided intrinsic self-calibration to effectively mitigate signal variations. Furthermore, a linear correlation was established for ratiometric fluorescence signal across the range of 0.25-35 μM (R2 = 0.999), and similarly for colorimetric absorbance (R2 = 0.999). This method provided an accurate, specific, sensitive and high-throughput detection of dopamine in human serum.
