Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of exercise rehabilitation and behavioral interaction nursing on postoperative quality of life and psychological outcomes in lung cancer patients.

Frontiers in oncology·2026
Same author

FgMob1 is involved in mitotic exit and regulates the development, abiotic stress response, and pathogenicity of <i>Fusarium graminearum</i>.

Frontiers in plant science·2026
Same author

The interplay of sleep architecture and exercise in executive function of middle-aged and older adults.

Frontiers in neurology·2026
Same author

Mechanism of toxicity of TiO<sub>2</sub> nanoparticles exposure on restraining bone growth of young rats: acting on HDAC9 nucleocytoplasmic translocation-mediated p53 deacetylation involving in growth plate chondrocyte differentiation and ferroptosis.

Chemico-biological interactions·2026
Same author

Molecular signatures and lineage diversification of neurogenic and gliogenic radial glia in the gyrencephalic ferret cortex.

Communications biology·2026
Same author

Unraveling Vps1-mediated endolysosomal sorting as a potential target for effective fungal disease control.

The New phytologist·2026
Same journal

Patterns of Body Composition Changes and Their Predictors During Cancer Therapy in Newly Diagnosed Adult Oncology Patients With Varying Baseline Nutritional Status at a Tertiary Hospital in Ethiopia: A Prospective Cohort Study.

Cancer reports (Hoboken, N.J.)·2026
Same journal

Advancing Cancer Cachexia Drug Development: Leveraging Biomarkers and Functional Endpoints to Optimize Trial Design.

Cancer reports (Hoboken, N.J.)·2026
Same journal

Salvage Therapy With Inotuzumab Ozogamicin in Relapsed/Refractory B-ALL After CAR-T Therapy and HSCT: A Case Series.

Cancer reports (Hoboken, N.J.)·2026
Same journal

Development of a Tumor-Bearing Animal Model to Evaluate Chemotherapy Efficacy and Toxicity.

Cancer reports (Hoboken, N.J.)·2026
Same journal

CCT7 Expression Affects the Prognosis of Lung Adenocarcinoma.

Cancer reports (Hoboken, N.J.)·2026
Same journal

Pembrolizumab-Induced Myositis: Diagnostic and Therapeutic Challenges From Two Case Reports and a Narrative Review.

Cancer reports (Hoboken, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jan 10, 2026

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines
08:42

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines

Published on: July 19, 2019

10.7K

Comparison of Gastric Cancer Models Using Different Dimensions In Vitro.

Wenhui Zheng1, Yubiao Lin1, Lulin Ji2

  • 1Oncology Department, Xiamen Haicang Hospital, Xiamen, China.

Cancer Reports (Hoboken, N.J.)
|November 27, 2025
PubMed
Summary
This summary is machine-generated.

Three-dimensional gastric cancer (GC) models, including spheroids and organoids, better mimic human tumors than 2D cultures. Organoids show higher growth rates and drug resistance, while spheroids exhibit the lowest drug sensitivity, informing GC model development.

Keywords:
drug testgastric cancerorganoidspheroidviability assay

More Related Videos

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds
13:34

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

Published on: April 6, 2016

10.6K
A Biomimetic Model for Liver Cancer to Study Tumor-Stroma Interactions in a 3D Environment with Tunable Bio-Physical Properties
08:40

A Biomimetic Model for Liver Cancer to Study Tumor-Stroma Interactions in a 3D Environment with Tunable Bio-Physical Properties

Published on: August 7, 2020

6.7K

Related Experiment Videos

Last Updated: Jan 10, 2026

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines
08:42

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines

Published on: July 19, 2019

10.7K
A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds
13:34

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

Published on: April 6, 2016

10.6K
A Biomimetic Model for Liver Cancer to Study Tumor-Stroma Interactions in a 3D Environment with Tunable Bio-Physical Properties
08:40

A Biomimetic Model for Liver Cancer to Study Tumor-Stroma Interactions in a 3D Environment with Tunable Bio-Physical Properties

Published on: August 7, 2020

6.7K

Area of Science:

  • Oncology
  • Biomedical Engineering
  • Cancer Research

Background:

  • Gastric cancer (GC) is a prevalent malignancy often diagnosed at late stages.
  • There is a critical need for advanced experimental models to improve drug efficacy, personalize treatment, and understand GC's molecular pathology.

Purpose of the Study:

  • To compare 2D, spheroid, and organoid models of gastric cancer.
  • To evaluate growth characteristics, drug sensitivity, and molecular mechanisms in different GC culture models.
  • To advance the development of more effective gastric cancer models.

Main Methods:

  • Utilized four gastric cancer cell lines and patient-derived tumor cells (PDTCs).
  • Assessed cell growth, drug sensitivity, and performed RNA-sequencing analyses.
  • Compared 2D cultures, spheroids, and organoids.

Main Results:

  • 3D models (spheroids and organoids) more closely resemble human tumor tissue structure than 2D models.
  • Organoids exhibited the highest growth rate, linked to PI3K/PTEN pathway activation and reduced apoptosis.
  • Spheroids showed the lowest drug sensitivity and highest expression of metabolic-related molecules, indicating increased drug resistance compared to organoids and 2D models.

Conclusions:

  • Significant differences exist among 2D, spheroid, and organoid models regarding growth, structure, apoptosis, signaling pathways, and drug sensitivity.
  • These findings highlight the distinct biological behaviors of GC cells in different culture systems.
  • The study provides crucial insights for selecting and developing optimal gastric cancer models for research and therapeutic development.