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Updated: Jan 10, 2026

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
Published on: October 15, 2015
Sidestream sulfide interference achieves mainstream nitritation: Co-extinction-differential recovery mechanisms of
Qingteng Gong1, Wei Zeng1, Qingan Meng1
1National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China.
Abstract:
Sustaining stable nitrite accumulation in municipal wastewater treatment remains a persistent challenge. In this study, we propose a novel nitrite accumulation strategy based on sidestream sulfide interference. By implementing a sidestream sulfide treatment unit for intermittent mainstream sludge cycling treatment, the mainstream process achieved 96.0 % nitrite accumulation rate. Sidestream sulfide treatment caused significant transcriptional suppression of amoA (43.3-63.9 % reduction) and nxrB (89.3-94.9 % reduction) genes during the mainstream aerobic stage, accompanied by synchronous inhibition of ammonia-oxidizing bacteria (AOB) (Nitrosomonas) and nitrite-oxidizing bacteria (NOB) (Nitrospira) translational activities. Crucially, AOB exhibited faster reactivation than NOB, as evidenced by the shorter doubling time of the amoA gene (8.7 days) compared to nxrB (24.1 days). This divergence in recovery kinetics facilitated rapid partial nitrification (PN) establishment. Overall, this work presents a sidestream sulfide-mediated strategy for accelerated mainstream PN initiation and provides multi-level mechanistic insights into microbial transcriptional-translational dynamics during process optimization.
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