Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

1.3K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Size of Biomolecular Condensates Dictates Fate in Liquid-Solid Phase Transitions through Amorphous-Amyloid Competition.

Journal of the American Chemical Society·2026
Same author

Lipids Contribute to Heterochromatin Condensation Revealed by Quantitative Raman-Brillouin Microscopy.

JACS Au·2026
Same author

Liquid-liquid phase separation-assisted Raman microscopy for sensitive and label-free analysis of enzymatic reactions and protein-small molecule interactions.

The Analyst·2025
Same author

Air-Stable Tetrazene Radical Cation Salts: Structural Requirements and Oxidation Catalysts.

Journal of the American Chemical Society·2025
Same author

Ca<sup>2+</sup>-driven PDIA6 biomolecular condensation ensures proinsulin folding.

Nature cell biology·2025
Same author

Visualizing Thiol Stress Responses in Cells with a Water-Soluble Raman Sensor.

Analytical chemistry·2025

Related Experiment Video

Updated: Jan 10, 2026

Chemical Dimerization-Induced Protein Condensates on Telomeres
08:52

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

3.6K

RNA-Mediated Inhibition Mechanism of Liquid-Liquid Phase Separation and Subsequent Aggregation Revealed by Raman

Taisei Ogura1, Uchu Matsuura1, Masato Machida1

  • 1Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba-Ku, Sendai 980-8578, Japan.

JACS Au
|November 28, 2025
PubMed
Summary

Short RNAs stabilize liquid-droplets of FUS proteins, preventing aggregation linked to neurodegenerative diseases like ALS. Endogenous RNAs also protect cells by regulating these droplets, highlighting RNA

Keywords:
Liquid−liquid phase separationNeurodegenerative diseasesProtein aggregationRNARaman microscopy

More Related Videos

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
12:58

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

Published on: September 12, 2019

10.2K
Combining Microfluidics and Microrheology to Determine Rheological Properties of Soft Matter during Repeated Phase Transitions
11:38

Combining Microfluidics and Microrheology to Determine Rheological Properties of Soft Matter during Repeated Phase Transitions

Published on: April 19, 2018

8.4K

Related Experiment Videos

Last Updated: Jan 10, 2026

Chemical Dimerization-Induced Protein Condensates on Telomeres
08:52

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

3.6K
Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
12:58

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

Published on: September 12, 2019

10.2K
Combining Microfluidics and Microrheology to Determine Rheological Properties of Soft Matter during Repeated Phase Transitions
11:38

Combining Microfluidics and Microrheology to Determine Rheological Properties of Soft Matter during Repeated Phase Transitions

Published on: April 19, 2018

8.4K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Neuroscience

Background:

  • Liquid-liquid phase separation (LLPS) of proteins like FUS drives aggregation in neurodegenerative diseases.
  • RNA's role in regulating FUS LLPS and aggregation is poorly understood.
  • The in-cell function of endogenous RNAs in FUS droplet dynamics is unknown.

Purpose of the Study:

  • Investigate RNA features influencing FUS droplet entry and aggregation inhibition.
  • Elucidate the molecular mechanisms of RNA-mediated FUS aggregation suppression.
  • Determine the role of endogenous RNAs in intracellular FUS droplet regulation.

Main Methods:

  • Raman microscopy for quantitative analysis of RNA-FUS interactions.
  • In vitro studies using buffer solutions with varying RNA lengths.
  • In vivo Raman imaging of FUS droplets in living cells.

Main Results:

  • RNA length is critical: short RNAs (<50 nt) concentrate in FUS droplets, inhibiting aggregation via electrostatic interactions.
  • Long RNAs (>1000 nt) dissolve FUS droplets, increasing FUS solubility.
  • Intracellular FUS droplets contain endogenous RNAs, similar to in vitro findings, and remain fluid, suppressing aggregation.

Conclusions:

  • Short RNAs stabilize FUS droplets by competing with FUS-FUS aggregation-driving interactions.
  • Long RNAs promote FUS solubility and droplet dissolution.
  • Endogenous RNAs play a vital protective role against pathogenic protein aggregation in cells.