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Updated: Jan 10, 2026

A Platform of Anti-biofilm Assays Suited to the Exploration of Natural Compound Libraries
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Biofilm comes back: Controlling regrowth by mitigating the cell-matrix interaction.

Yu-Heng Deng1, Joo Hun Lee1, Myung-Joo Kim2

  • 1Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana- Champaign Urbana IL United States.

Chemical Engineering Journal (Lausanne, Switzerland : 1996)
|November 28, 2025
PubMed
Summary

A novel strategy using manganese dioxide-doped diatoms effectively breaks down biofilm extracellular polymeric substances (EPS), preventing bacterial regrowth and inhibiting biofilm formation for extended periods.

Keywords:
BiofilmBiofilmQEPSMicrobubblerRegrowth

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Area of Science:

  • Microbiology and Materials Science
  • Antimicrobial resistance and biofilm control strategies

Background:

  • Biofilms cause significant issues in infections and sanitation.
  • Existing antimicrobial agents struggle to prevent biofilm reformation after treatment.

Purpose of the Study:

  • To investigate the role of extracellular polymeric substances (EPS) in biofilm regrowth after treatment.
  • To develop an advanced strategy to inhibit biofilm reformation.

Main Methods:

  • Quantified cell-EPS interaction using Manders coefficient and EPS mass per cell via BiofilmQ software.
  • Developed a sequential treatment using MnO2-doped diatoms to reduce EPS volume prior to H2O2 + PAA application.

Main Results:

  • Hydrogen peroxide (H2O2) and peracetic acid (PAA) mixture increased EPS mass per cell, contributing to regrowth.
  • The sequential strategy significantly reduced EPS mass associated with cells.
  • Biofilm regrowth was inhibited for over 2 months using the novel approach.

Conclusions:

  • EPS plays a critical role in bacterial cell survival and biofilm reformation post-treatment.
  • A sequential strategy involving EPS reduction is effective in long-term biofilm inhibition.
  • This approach offers a promising solution for controlling infections and biofouling in various applications.