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Related Experiment Video

Updated: Jan 10, 2026

Generation of Orthotopic Pancreatic Tumors and Ex vivo Characterization of Tumor-Infiltrating T Cell Cytotoxicity

Generation of Orthotopic Pancreatic Tumors and Ex vivo Characterization of Tumor-Infiltrating T Cell Cytotoxicity

Published on: December 7, 2019

Bioengineered Pancreatic Cancer Immunosuppressive Microenvironment Models for Screening Immunotherapies.

Maria V Monteiro^1,2, Margarida Henriques-Pereira^1,2, Bruno M Neves³

¹Department of Chemistry, CICECO - Aveiro Institute of Materials, University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal.

Advanced Healthcare Materials

|November 28, 2025

Summary

This summary is machine-generated.

Researchers developed novel bioengineered pancreatic cancer models to mimic tumor complexity. These models effectively recapitulate immunosuppressive features and show promise for screening new immunotherapies against pancreatic cancer.

Keywords:

immunosuppressive microenvironment immunotherapy in vitro models pancreatic cancer tumor‐stroma interactions

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Last Updated: Jan 10, 2026

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Published on: December 7, 2019

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Flow Cytometry-Based Isolation and Therapeutic Evaluation of Tumor-Infiltrating Lymphocytes in a Mouse Model of Pancreatic Cancer

Flow Cytometry-Based Isolation and Therapeutic Evaluation of Tumor-Infiltrating Lymphocytes in a Mouse Model of Pancreatic Cancer

Published on: January 17, 2025

Area of Science:

Biomedical Engineering
Cancer Biology
Immunology

Background:

Pancreatic cancer exhibits resistance to treatment due to its dense stroma and immunosuppressive microenvironment.
Accurate in vitro models are crucial for developing effective immunotherapeutic strategies for pancreatic cancer.
Current models struggle to replicate the intricate features of the pancreatic tumor microenvironment.

Purpose of the Study:

To bioengineer advanced pancreatic cancer models that emulate the native tumor composition and immunosuppressive hallmarks.
To create a platform for evaluating immunotherapeutic strategies in a more realistic tumor microenvironment.
To investigate tumor-immune interplay within a bioengineered model.

Main Methods:

Development of miniaturized tumor-stroma platforms (cancer-on-a-bead) using biomaterials.
Co-culture of these platforms with immune cells (T cells, dendritic cells, M0 macrophages).
Assessment of key immunosuppressive signatures and immune cell function within the models.

Main Results:

The bioengineered models successfully recapitulated tumor immunosuppressive signatures, including suppressed antigen presentation and M2 macrophage polarization.
T cell exhaustion was observed, mirroring pancreatic cancer's in vivo characteristics.
Testing of anti-PD-1 antibody immunotherapy partially restored T cell function in the 3D tumor platforms.

Conclusions:

Compartmentalized tumor-stroma models offer a valuable preclinical tool for screening immunotherapeutics.
These advanced models can help overcome challenges in developing treatments for pancreatic cancer.
The developed cancer-on-a-bead platforms provide a more accurate representation of pancreatic cancer for drug discovery.