Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inorganic Nitrogen Assimilation01:22

Inorganic Nitrogen Assimilation

433
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
433
Sulfur Assimilation01:20

Sulfur Assimilation

301
Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to...
301

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Closed-loop hydrostannylation of white phosphorus using Bu<sub>3</sub>SnCl and NaBH<sub>4</sub>: one-pot access to organophosphorus compounds.

Dalton transactions (Cambridge, England : 2003)·2026
Same author

Coulombic control of charge transfer in radicals with quartet recycling luminescence.

Nature communications·2026
Same author

Crystallise, poise, capture: a multimodal platform for correlated structural and spectroscopic characterisation of redox enzymes.

Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry·2026
Same author

H<sub>2</sub>O<sub>2</sub> repurposes plant O<sub>2</sub> sensing to regulate post-hypoxia responses.

Nature·2026
Same author

Structural basis of the promiscuity of the unusual Fe(II) and 2-oxoglutarate dependent human aspartate/asparagine-β-hydroxylase.

Nature communications·2026
Same author

High-spin state dynamics and quintet-mediated emission in intramolecular singlet fission.

Nature communications·2026
Same journal

VOCs Adsorption and Exchange Properties in Bispidine-Based Mn(II) 1D CPs Made of Orthogonally Oriented Linear Chains.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Electrosynthesis of Glycine From Nitrate and Glyoxylic Acid Over a Bi<sub>2</sub>S<sub>3</sub> Nanosheets-Based Catalyst.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Symmetry Breaking in Achiral Porphyrins: Noncovalent Origins of Emergent Optical Activity.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Modulation of O<sub>2</sub> Affinity and Enzymatic Activity of Core‒Shell Structured Hemoglobin Nanoparticles.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Stepwise Synthesis of Tetrabenzotriazaporphyrins (TBTAPs) and Their Open 2- and 3-Ring Fragments.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Geometry-Based Neural-Network Prediction of Electron Localization Function Topology in Dense Hydrogen.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
See all related articles

Related Experiment Video

Updated: Jan 10, 2026

Author Spotlight: Characterizing Porous Materials for Aiding the Development of Robust Metal-Organic Frameworks with Adsorption Behavior
06:45

Author Spotlight: Characterizing Porous Materials for Aiding the Development of Robust Metal-Organic Frameworks with Adsorption Behavior

Published on: March 8, 2024

9.7K

Investigating N2 Fixation Using a Bulky Fe(bisphosphine)2 Framework.

Andrew D Crawford1, Laurence R Doyle1, Samuel J Horsewill2

  • 1Department of Chemistry, Imperial College London, London, UK.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|November 28, 2025
PubMed
Summary
This summary is machine-generated.

This study synthesized a sterically hindered iron dinitrogen complex, Fe(N2)(dibpe)2, for nitrogen fixation. While effective stoichiometrically, catalytic attempts failed due to sluggish kinetics and weak N2 binding in intermediates.

Keywords:
ammoniahydrazineironnitrogen fixationphosphine ligands

More Related Videos

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

6.5K
On-line Analysis of Nitrogen Containing Compounds in Complex Hydrocarbon Matrixes
07:49

On-line Analysis of Nitrogen Containing Compounds in Complex Hydrocarbon Matrixes

Published on: August 5, 2016

11.1K

Related Experiment Videos

Last Updated: Jan 10, 2026

Author Spotlight: Characterizing Porous Materials for Aiding the Development of Robust Metal-Organic Frameworks with Adsorption Behavior
06:45

Author Spotlight: Characterizing Porous Materials for Aiding the Development of Robust Metal-Organic Frameworks with Adsorption Behavior

Published on: March 8, 2024

9.7K
Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

6.5K
On-line Analysis of Nitrogen Containing Compounds in Complex Hydrocarbon Matrixes
07:49

On-line Analysis of Nitrogen Containing Compounds in Complex Hydrocarbon Matrixes

Published on: August 5, 2016

11.1K

Area of Science:

  • Inorganic Chemistry
  • Organometallic Chemistry
  • Catalysis

Background:

  • Nitrogen fixation is crucial for ammonia production.
  • Iron complexes offer potential for efficient nitrogen reduction.
  • Sterically hindered ligands can influence reactivity and selectivity.

Purpose of the Study:

  • To synthesize and characterize a highly hindered iron(0) dinitrogen complex, Fe(N2)(dibpe)2.
  • To assess its nitrogen fixation reactivity compared to less hindered analogues.
  • To investigate the reasons for limitations in catalytic nitrogen fixation.

Main Methods:

  • Synthesis and full characterization of Fe(N2)(dibpe)2.
  • Stoichiometric nitrogen fixation studies with acids.
  • Catalytic nitrogen fixation attempts using acids and reductants.
  • Mechanistic investigations including isolation and characterization of an Fe(I) intermediate.

Main Results:

  • Fe(N2)(dibpe)2 was successfully synthesized and characterized.
  • The complex achieved efficient stoichiometric N2 fixation to NH3 and N2H4.
  • Catalytic attempts resulted in poor performance.
  • Mechanistic studies revealed sluggish kinetics and weak N2 binding to the Fe(I) intermediate.

Conclusions:

  • Steric bulk in iron dinitrogen complexes does not inherently improve catalytic performance.
  • Weak N2 binding to the Fe(I) intermediate hinders catalytic cycle regeneration.
  • Further ligand design is needed to overcome kinetic limitations in iron-catalyzed nitrogen fixation.