Related Experiment Video
Updated: Jan 6, 2026

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production
Published on: March 2, 2014
Transcriptomic analysis reveals Rnd1 to be a key player in antiviral immunity against Akabane virus via the TNF-α
Dongjie Chen1, Jingjing Wang1, Chao Sun2
1Institute of Animal Inspection and Quarantine, Chinese Academy of Quality and Inspection & Testing, No. 11, Ronghuanan Road, Beijing, 100176, China.
Abstract:
Akabane virus (AKAV), the etiological agent of Akabane disease, is known to induce congenital malformations and neuropathologies in the fetuses of pregnant cattle and sheep. To analyze the changes in gene expression patterns that occur in AKAV-infected cells, two types of primary bovine cells - primary bovine testicular Sertoli cells (BTSCs) and primary bovine joint synovial cells (BJSCs) - were selected. These cells were confirmed to be infected by AKAV strain TJ2016. Subsequently, RNA-sequencing technology was employed to analyze the transcriptomic profiles of AKAV-infected BTSCs and BJSCs, as well as Madin-Darby ovine kidney (MDOK) cells. The AKAV-infected cells exhibited activation of antiviral signaling pathways. Notably, there was upregulation of the expression levels of interferon-stimulated genes, as well as genes related to inflammation and cytokines. IL-1β, TNF-α, CXCL8, CCL2, and Rnd1 were found to be significantly upregulated in AKAV-infected cells. Moreover, Rnd1 was found to inhibit the replication of AKAV. Because TNF-α plays an important role in the induction of Rnd1, this provides additional evidence for the regulation and function of Rnd1.
More Related Videos
12:43Monitoring Activation of the Antiviral Pattern Recognition Receptors RIG-I And PKR By Limited Protease Digestion and Native PAGE
Published on: July 29, 2014
06:44Confocal Imaging of Double-Stranded RNA and Pattern Recognition Receptors in Negative-Sense RNA Virus Infection
Published on: January 26, 2019
Related Concept Videos
Leaky Scanning
Immune Response Against Viral Pathogens
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The...
RNA Interference
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
The JAK-STAT Signaling Pathway
Viruses with RNA Genomes