Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.8K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.8K
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

4.1K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
4.1K
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

5.2K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
5.2K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.6K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

AI-guided analysis of human pancreatic islet sociology reveals distinct cell compositional changes in type 1 diabetes.

bioRxiv : the preprint server for biology·2026
Same author

Local control of dopamine release in nucleus accumbens gates opioid withdrawal aversion.

bioRxiv : the preprint server for biology·2026
Same author

Psychosis as a multisystem disorder of aberrant aging.

npj aging·2026
Same author

Quantification of Appetitive and Mechanical Aversive Associative Learning Paradigms in Drosophila via a Y-maze Assay.

Current protocols·2026
Same author

Unsupervised Identification of Protein Compositions and Conformations via Implicit Content-Transformation Disentanglement.

Proceedings. IEEE International Conference on Computer Vision·2026
Same author

Dopaminergic modulation of pancreatic beta-cell insulin secretion and implications for antipsychotic-induced glucose dysregulation: a systematic review and meta-analysis.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology·2026

Related Experiment Video

Updated: Jan 6, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

9.0K

Localization of macromolecules in crowded cellular cryo-electron tomograms from extremely sparse labels.

Mostofa Rafid Uddin1, Ajmain Yasar Ahmed2, H M Shadman Tabib3

  • 1Ray and Stephanie Lane Computational Biology Department, Carnegie Mellon University, 5000 Forbes Avenue, Pittsburgh, PA 15213, United States.

Briefings in Bioinformatics
|November 28, 2025
PubMed
Summary

TomoPicker efficiently localizes macromolecules in cryo-electron tomography (cryo-ET) images using minimal annotations. This novel method significantly reduces data requirements compared to traditional approaches, enabling faster in situ structural determination.

Keywords:
3D classificationcell and structural biologycryo-electron tomographymacromolecule localizationpositive-unlabeled learning

More Related Videos

Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows
09:53

Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows

Published on: September 13, 2021

7.5K
The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells
11:45

The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells

Published on: February 27, 2020

10.1K

Related Experiment Videos

Last Updated: Jan 6, 2026

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

9.0K
Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows
09:53

Micropatterning Transmission Electron Microscopy Grids to Direct Cell Positioning within Whole-Cell Cryo-Electron Tomography Workflows

Published on: September 13, 2021

7.5K
The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells
11:45

The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells

Published on: February 27, 2020

10.1K

Area of Science:

  • Structural biology
  • Cell biology
  • Biophysics

Background:

  • Accurate localization of macromolecules in cellular cryo-electron tomography (cryo-ET) is essential for determining their in situ structures.
  • Conventional template matching methods are limited by template bias and low throughput.
  • The scarcity of annotated data presents a significant hurdle for developing effective learning-based localization methods.

Purpose of the Study:

  • To develop an annotation-efficient method for macromolecule localization in tomograms.
  • To address the challenges posed by limited annotated data in cryo-ET image analysis.
  • To improve the throughput and accuracy of macromolecule structure determination.

Main Methods:

  • TomoPicker treats macromolecule localization as a voxel classification problem.
  • The method employs two distinct positive-unlabeled learning strategies.
  • Evaluation was performed on cryo-ET datasets from eukaryotic and prokaryotic cells.

Main Results:

  • TomoPicker achieved performance comparable to state-of-the-art supervised methods using only 10 annotations.
  • The method demonstrated up to 98% reduction in data requirements compared to supervised learning.
  • Significant improvements were observed over existing methods under sparse annotation conditions.

Conclusions:

  • TomoPicker offers a highly efficient solution for macromolecule localization in cryo-ET.
  • The annotation-efficient approach democratizes in situ structural biology by reducing data burden.
  • This method facilitates more accessible and rapid structural determination of cellular components.