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Updated: Jan 10, 2026

Author Spotlight: Designing Simple and Inexpensive Techniques to Grow Methane-Oxidizing Bacteria in the Laboratory
Published on: September 6, 2024
Quorum sensing signaling molecules mitigate formic acid stress in syntrophic propionate oxidation: Enhancing
1School of Chemistry and Life Resources, Renmin University of China, Beijing 100872, PR China.
Abstract:
In anaerobic digestion (AD) system, high concentrations of formic acid inhibit the electron transfer efficiency of the short-chain fatty acid syntrophic oxidation process, ultimately affecting methanogenic efficacy. Thus, we investigated whether quorum sensing (QS) regulator N-acyl homoserine lactones (AHLs) can alleviate the stress effect of formic acid on syntrophic oxidizing bacteria and promote electron transfer. Under 15 mM formic acid stress, C6-HSL enhanced maximum CH4 production (2.0 μg/L, 11 days, p < 0.05), restored succinate dehydrogenase and malate dehydrogenase activities to 59.9 %-80.6 % and 70.8 %-80.0 % of the initial levels. Adding C4-HSL restored the ETS activity to 63.8 %-74.9 % of the initial level. Adding AHLs can accelerate the efficiency of interspecies electron transfer by activating the QS system, and enhance EPS synthesis to enhance cellular protective barrier and alleviate microbial toxicity. The results provided a new strategy for the regulation of the AD system under high acid stress.
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