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Construction of an mKate-based fluorescent protein reporting system in Limosilactobacillus reuteri

Yihang Wang1, Xinxin Wang1, Daoyan Wu1

  • 1Guizhou Key Laboratory of Microbiome and Infectious Disease Prevention and Control, Guizhou Medical University, Guiyang, Guizhou 561113, China; Key Laboratory of Ministry of Education for Endemic and Ethnic Diseases and Key Laboratory of Medical Molecular Biology of Guizhou, Guizhou Medical University, Guiyang, Guizhou 561113, China.

Journal of Microbiological Methods
|November 28, 2025
PubMed

Keywords:
Expression regulationFluorescent proteinGenetic engineeringLimosilactobacillus reuterimKate

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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...