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Updated: Jan 10, 2026
RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord
Published on: November 1, 2014
Transcriptome profile analysis of miRNA and mRNA in chicken embryo gonad sex differentiation
Endashaw Jebessa1, Yanhua He1, Hao Qu1
1State Key Laboratory of Swine and Poultry Breeding Industry & Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition & Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China.
Abstract:
Despite the integrative function of miRNAs and genes in chicken embryonic gonadal sex differentiation, their roles remain poorly understood. Here, we used RNA-seq to analyze 12 gonadal samples from embryonic days 5 (E5) and 9 (E9). Our analysis of miRNA and mRNA expression during this key developmental stage revealed five DEmiRNAs and 235 DEmRNAs consistently associated with left-right asymmetry in female gonads in both F5L vs F5R and F9L vs F9R comparisons. The interaction between DEmiRNAs and target DEmRNAs in the left-right asymmetry of embryonic gonadal development at E5 and E9 in chickens, along with the KEGG pathway enrichment analysis, revealed the involvement of several significant pathways. These include the complement and coagulation cascades, tight junctions, and synthesis and secretion of aldosterone. Differentially expressed miRNAs and target genes at embryonic days 5 and 9 reveal key molecular mechanisms driving gonadal sex differentiation in chickens.
