In silico characterization of the OSBPL6 gene and its potential role in ascites syndrome in broiler chickens

Ascites syndrome is a multifactorial metabolic disorder in broiler chickens, characterized by pulmonary hypertension, fluid accumulation, and high mortality, leading to significant economic losses. The oxysterol-binding protein-like 6 gene (OSBPL6) has been associated with ascites susceptibility, but its molecular role remains unclear. Therefore, this study was aimed to perform a comprehensive in-silico analysis of chicken OSBPL6, including analyses of physicochemical properties, structural features, post-translational modifications, protein-protein interactions, promoter motifs, and tissue-specific expression to elucidate its involvement in ascites syndrome pathogenesis in broiler chickens. OSBPL6 encodes a 978-amino acid protein with a molecular mass of 110,905.88 Da, exhibiting predominantly hydrophilic characteristics (GRAVY = -0.583), moderate thermostability (aliphatic index = 76.45), and predicted instability in vitro (instability index = 65.47). Analysis revealed 134 phosphorylation sites and 6 glycosylation sites, indicating extensive post-translational regulation. Subcellular localization predictions suggested cytoplasmic residence with peripheral membrane associations and potential nucleocytoplasmic shuttling capability. Conserved PH, ORD, and FFAT domains were identified, supporting roles in sterol transport and lipid binding functions. Among structural models generated, trRosetta showed the highest reliability (QMEANDisCo =0.66, ERRAT = 94.3 %). Protein-protein interaction analysis linked OSBPL6 to pathways related to cardiac hypertrophy, muscle metabolism, and oxidative stress responses. Promoter analysis identified transcription factor binding sites linked to vascular remodeling, myocardial hypertrophy, and muscle development. Expression profiling showed highest OSBPL6 expression in skeletal muscle and elevated levels in cardiac tissue, consistent with ascites-associated pathophysiology. This integrative analysis highlights OSBPL6 as a candidate regulator of muscle growth and cardiopulmonary remodeling, with potential as a biomarker for genetic selection against ascites in broiler chickens.