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Cost-effective immobilization of Chaetomium erraticum dextranase on c-MWCNT
Barzan Ismael Ghafour1, Yakup Aslan1
1Department of Food Engineering, Faculty of Engineering, Siirt University, Kezer Campus, 56100 Siirt, Turkey.
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In this study, dextranase enzyme (CED) from Chaetomium erraticum was immobilized on carboxylated multi-walled carbon nanotubes (c-MWCNTs) via adsorption. Immobilization conditions were optimized, and both free (FCED) and immobilized enzymes (ICED) were characterized. The process achieved 100 % immobilization efficiency and 114.13 % activity recovery. After immobilization, the optimum pH shifted from 5.0 to 6.0, while the optimum temperature remained constant at 55 °C. The Km value was identical for both enzymes (54.35 g/L), but ICED exhibited a higher Vmax than FCED. ICED retained its activity after 20 reuses and 30 days of storage. Moreover, isomaltooligosaccharide (IMO) production by ICED was completed in 10 h. The results demonstrate that this approach enables simple, rapid, and cost-effective enzyme immobilization suitable for industrial IMO production.
