Related Experiment Video
Updated: Jan 9, 2026

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
Published on: July 7, 2020
Equisetin targets membrane integrity and transcriptional networks to combat multidrug-resistant Enterococcus faecalis
Xiao-Le Han1, Jiang Chen2, Tao Zhou1
1Key Laboratory of Catalysis and Energy Materials Chemistry of Ministry of Education & Hubei Key Laboratory of Catalysis and Materials Science, School of Chemistry and Materials Science, South-Central Minzu University, Wuhan, 430074, PR China.
Abstract:
This study evaluates the natural product Equisetin as a potent agent against multidrug-resistant Enterococcus faecalis (MDR E. faecalis). Equisetin exhibited strong antibacterial activity (MIC = 5.84 μg/mL) by disrupting membrane integrity, inducing protein/DNA leakage, depolarizing membrane potential, elevating ROS, and depleting ATP. It also inhibited biofilm formation and showed minimal hemolytic activity without inducing resistance. Transcriptomics revealed upregulation of DNA repair/stress genes and downregulation of metabolic/protein synthesis genes, indicating a multi-target mechanism. These findings position Equisetin as a promising candidate for treating MDR infections.
Related Concept Videos
Gene Regulation in Microbial Communities: Quorum Sensing
Development of Antibiotic Resistance
Antimicrobial Effectiveness
Stringent Response in E. coli
Antiepileptic Drugs: Potassium Channel Activators
Ezogabine has gained approval as an adjunctive treatment...
The Electron Transport Chain
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...

