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Updated: Feb 9, 2026
In vivo Evaluation of Mucociliary Clearance in Mice
Published on: December 18, 2020
Precision of single injection (51Cr)EDTA plasma clearance and endogenous creatinine clearance determinations in
Insights
Total [51Cr]EDTA plasma clearance (E) is more reliable than 24-hour endogenous creatinine clearance (C) for routine pediatric glomerular filtration rate (GFR) assessment. Method E demonstrated higher precision and accuracy in children across various GFR levels.
Area of Science:
- Pediatric Nephrology
- Renal Physiology
- Diagnostic Accuracy
Background:
- Glomerular filtration rate (GFR) is a key indicator of kidney function in children.
- Accurate GFR measurement is crucial for diagnosing and managing renal diseases.
- Existing clearance methods have varying degrees of precision and accuracy.
Purpose of the Study:
- To compare the precision of two routine GFR assessment methods in children.
- To evaluate total [51Cr]EDTA plasma clearance (E) versus 24-hour endogenous creatinine clearance (C).
- To determine the more reliable method for pediatric GFR monitoring.
Main Methods:
- Investigated total [51Cr]EDTA plasma clearance (E) using a simplified single injection method.
- Assessed 24-hour endogenous creatinine clearance (C), facing challenges with accurate urine collection.
- Analyzed precision based on total day-to-day variation in 31 children (0.6-14 years).
Main Results:
- Method C succeeded in only 20/31 children due to urine collection issues.
- For E ≥ 80 ml/min/1.73 m2: E precision was 5.5%, C precision was 13.8%.
- For E < 80 ml/min/1.73 m2: E precision was 7.8%, C precision was 20.8%.
Conclusions:
- Total [51Cr]EDTA plasma clearance (E) shows significantly higher precision than creatinine clearance (C) in children.
- Literature suggests higher inaccuracy in predicting GFR from C compared to E.
- Method E is recommended as a more reliable option for routine pediatric GFR assessment.
Abstract:
The precision of two different clearance methods as used for routine assessment of the glomerular filtration rate (GFR) was investigated in thirty-one children aged 0.6-14 years: total [51Cr]EDTA plasma clearance (E) determined by a simplified single injection method; and 24 h endogenous creatinine clearance (C). Determination of C twice only succeeded in twenty children because of problems in collecting 24 h urine accurately. The precision (determined from the total day-to-day variation) for single determinations in patients with E greater than or equal to 80 ml/min/1.73 m2 was 5.5% for E and 13.8% for C. The corresponding figures for E less than 80 ml/min/1.73 m2 was 7.8% for E and 20.8% for C. Data in the literature on the inaccuracies of C and E versus GFR suggest that the degree of inaccuracy in predicting GFR from C is much higher than that from E, a feature which together with the present findings on precision indicates that E is much more reliable than C for routine determination of GFR in children.
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