Related Experiment Video

Updated: Feb 7, 2026

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis
08:44

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis

Published on: September 7, 2022

2.9K

Single injection (51Cr)EDTA plasma clearance determination in children using capillary blood samples

J Brochner-Mortensen, J Christoffersen

    Scandinavian Journal of Clinical and Laboratory Investigation
    |November 1, 1977
    PubMed

    Insights

    Determining glomerular filtration rate using [51Cr]EDTA plasma clearance with capillary blood samples is reliable in children. This method offers accurate and precise results, simplifying the assessment of renal function.

    Area of Science:

    • Pediatric Nephrology
    • Nuclear Medicine
    • Clinical Chemistry

    Background:

    • Glomerular filtration rate (GFR) is a key indicator of kidney function.
    • Accurate GFR measurement in children is crucial for diagnosis and management.
    • Traditional methods often require venous blood samples, which can be challenging in pediatric populations.

    Purpose of the Study:

    • To evaluate the reliability of a simplified single injection method for determining total [51Cr]EDTA plasma clearance (E) using capillary blood samples in children.
    • To compare the accuracy and precision of GFR measurements obtained from capillary versus venous blood samples.

    Main Methods:

    • Twenty children underwent a simplified single injection of [51Cr]EDTA (4.5 μCi/kg body weight).
    • Plasma clearance (E) was determined using small-volume (0.2 ml) capillary blood samples.
    • Simultaneous measurements were performed using venous blood samples for comparison.
    • Reproducibility was assessed by day-to-day variation in E measurements.

    Main Results:

    • [51Cr]EDTA plasma clearance values derived from capillary blood samples showed no significant difference compared to those from venous blood samples (mean ratio ± SD: 1.02 ± 0.06).
    • The reproducibility of E determination using capillary blood was high, with a total day-to-day variation of 6.7% in children with decreased renal function and 6.9% in those with normal renal function.
    • The findings demonstrate excellent agreement and precision between the two sampling methods.

    Conclusions:

    • The simplified single injection method using capillary blood samples is an accurate and precise approach for determining [51Cr]EDTA plasma clearance (E) in children.
    • This method offers a practical and reliable alternative for assessing GFR in pediatric patients, potentially reducing patient discomfort.
    • Capillary blood sampling enhances the feasibility of routine GFR assessment in pediatric nephrology.

    More Related Videos

    Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients08:25

    Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients

    Published on: September 9, 2020

    11.8K
    Oral Biofilm Sampling for Microbiome Analysis in Healthy Children10:42

    Oral Biofilm Sampling for Microbiome Analysis in Healthy Children

    Published on: December 31, 2017

    17.9K

    Related Concept Videos

    Composition of Blood Plasma01:24

    Composition of Blood Plasma

    Blood plasma is a fluid that contains approximately 92% water and 8% solutes. The solutes include various types of proteins, which constitute about 7% of the total solutes in the plasma. The high-molecular-weight proteins—albumins, globulins, and fibrinogen—are essential to plasma function. Albumins, making up about 60% of the plasma proteins, maintain the osmotic balance within blood vessels by preventing excessive water leakage. Additionally, albumins serve as carrier proteins,...
    8.6K
    Protein Buffers in Blood Plasma and Cells01:20

    Protein Buffers in Blood Plasma and Cells

    The human body utilizes protein buffer systems to maintain a stable pH. These systems capitalize on the dual role of amino acids, which can act as acids or bases by accepting or releasing hydrogen ions in response to pH changes. Protein buffer systems are particularly significant in the extracellular fluid (ECF) and intracellular fluid (ICF) of active cells, where structural and functional proteins provide substantial buffering capacity.
    Certain amino acids can exist in a zwitterion state at a...
    3.9K
    Factors Affecting Renal Clearance: Drug's Physicochemical Properties and Plasma Levels01:31

    Factors Affecting Renal Clearance: Drug's Physicochemical Properties and Plasma Levels

    Renal clearance of a drug is influenced by various factors, including its physicochemical properties and plasma levels. These factors play a significant role in determining how efficiently the kidneys eliminate a drug.
    One important factor is the drug's molecular size. The kidneys readily excrete smaller molecules below 300 Daltons (Da). On the other hand, molecules weighing between 300 and 500 Da are excreted through both urine and bile. Larger molecules above 500 Da tend to be excreted...
    601
    Determination of Renal Drug Clearance: Graphical and Midpoint Methods01:07

    Determination of Renal Drug Clearance: Graphical and Midpoint Methods

    Renal clearance, a crucial parameter in pharmacokinetics, can be determined using two different methods: the graphical method and the midpoint method. These methods provide insights into the rate of drug excretion by the kidneys and aid in assessing renal function.
    The graphical method involves plotting the rate of drug excretion in urine against the plasma drug concentration. By analyzing the graph, the clearance can be calculated and obtained. Drugs rapidly excreted by the kidneys exhibit a...
    401
    EDTA: Chemistry and Properties01:22

    EDTA: Chemistry and Properties

    Polydentate ligands are most widely used in complexometric titrations because they form more stable complexes with the metal ions than mono- or bidentate ligands due to the chelate effect. Examples of polydentate ligands are ethylenediaminetetraacetic acid (EDTA), crown ethers, and cryptands. The most important feature of optimal polydentate ligands is the ability to form 1:1 complexes in a single-step process. Amino carboxylic acid derivatives are frequently used as complexing agents. EDTA is...
    3.4K
    Gas Chromatography: Sample Injection Systems01:08

    Gas Chromatography: Sample Injection Systems

    In gas chromatography, the sample is introduced as a vapor plug into the carrier gas stream for high efficiency and resolution. A microsyringe injects the sample solution into a heated sample port, vaporizing it and mixing it with the carrier gas. This process is important to ensure the sample is properly prepared for analysis. Thermally sensitive samples can be injected directly into the column and volatilized by slowly increasing the column temperature.
    Two primary injection methods are used...
    1.6K