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A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells

Published on: April 11, 2014

Simultaneous total antioxidant capacity assessment and ascorbic acid accurate quantification utilizing a

Yanli Li¹, Feng Cao², Xiaoyu Deng²

¹Sichuan Engineering Research Center for Molecular Targeted Diagnostic & Therapeutic Drugs, Department of Chemistry, Xihua University, Chengdu 610039, China.

|November 30, 2025

Summary

Researchers developed novel iron-modified carbon dots (Fe-CDs) for a dual-mode sensor array. This array accurately measures total antioxidant capacity (TAC) in food and biological samples, advancing nutritional analysis and safety.

Keywords:

Ascorbic acid Carbon dots Oxidase-like activity Sensor array Total antioxidant capacity

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A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes

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A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes

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Published on: June 10, 2022

Area of Science:

Analytical Chemistry
Materials Science
Biotechnology

Background:

Analyzing antioxidants in food is challenging due to similar structures.
Dual-mode sensor arrays offer a promising solution for complex sample analysis.
Existing methods may require hydrogen peroxide, limiting applications.

Purpose of the Study:

To develop novel oxidase-like carbon dots (Fe-CDs) for enhanced catalytic activity.
To construct a hydrogen peroxide-independent colorimetric-fluorescent sensor array.
To selectively identify and quantify total antioxidant capacity (TAC) in diverse samples.

Main Methods:

Synthesized Fe-surface modified carbon dots (Fe-CDs) with enhanced oxidase-like activity.
Developed a dual-mode (colorimetric-fluorescent) sensor array utilizing Fe-CDs.
Applied the sensor array to analyze TAC in fruit juices and differentiate cell types.

Main Results:

Fe-CDs exhibited significantly enhanced oxidase-like activity due to Fe-oxygen coordination.
The sensor array successfully discriminated TAC levels in five common fruit juices.
The system accurately detected ascorbic acid TAC with low detection limits (0.9 μM) and wide linear ranges (2.5-60 μM).

Conclusions:

Novel Fe-CDs offer a robust platform for developing advanced biosensors.
The H2O2-independent sensor array provides a versatile tool for food nutrition assessment.
This technology holds potential for in vitro diagnostics and safety monitoring applications.