Rapid, Reliable, and Green: A Novel RP-HPLC Method for Quercetin Estimation in Green-Synthesized Nanoparticles

Area of Science:

• Analytical Chemistry
• Nanotechnology
• Pharmacognosy

Background:

• Quercetin (QUE) is a flavonoid with medicinal properties found in plants.
• Green synthesis of nanoparticles using plant extracts is an emerging eco-friendly technology.
• Accurate quantification of QUE in nanoparticles is crucial for its pharmaceutical applications.

Purpose of the Study:

• To develop a facile, accurate, and reliable reverse-phase high-performance liquid chromatography (RP-HPLC) method for QUE determination in nanoparticles.
• To validate the developed method according to ICH guidelines.
• To highlight the method's novelty in achieving faster QUE detection.

Main Methods:

• Utilized a C18 column for chromatographic separation.
• Employed a mobile phase of methanol and 0.1% orthophosphoric acid (OPA) (80:20, v/v).
• Optimized flow rate to 1.0 mL/min and detection wavelength to 371 nm.

Main Results:

• Achieved high linearity (R² > 0.999) in the range of 0.1-8.0 μg/mL.
• Determined a retention time of 3.5 min for QUE.
• Established LOD at 0.6 μg/mL and LOQ at 2.1 μg/mL, with validation parameters meeting ICH guidelines.

Conclusions:

• The developed RP-HPLC method is suitable for accurate and reliable quantification of QUE in nanoparticles.
• The method offers a significant advantage with a reduced retention time compared to conventional techniques.
• This advancement supports the use of plant-derived nanoparticles in pharmaceutical research and development.