A Comparative Ultracentrifugation Protocol for Isolating, Purifying, Selecting, and Characterizing Mouse and Human...

Nanovesicles, particularly exosomes, are crucial in cellular communication across physiological, biological, and pathological contexts. The processes of isolation, purification, and characterization are therefore vital to understanding their biological roles and therapeutic potential. This chapter provides a comprehensive overview of current techniques for isolating and purifying exosomes derived from healthy cells, especially stem cells and cancer cells, including an analysis of the challenges, advantages, and limitations associated with each method. Additionally, a selection process is outlined to serve as a guide for enriching functionally diverse exosome vesicles. The chapter aims to assist researchers in selecting the most appropriate exosome isolation method to align with their research objectives.