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Updated: Jan 9, 2026
Author Spotlight: Advancing Pathogen Detection and Disease Assessment in Real-Time Using M-ROSE
Published on: March 1, 2024
"ROSE" Revealing Its True Beauty-Comparing the Efficacy of Rapid On-Site Evaluation (ROSE) in Determining Specimen
Hira Nasir1, Fizza Jahangir1, Muhammad Ahsan1
1Department of Histopathology, Chughtai Institute of Pathology, Lahore, Pakistan.
Introduction:
Fine needle aspiration cytology (FNAC) is widely used to evaluate various lesions. Rapid on-site evaluation (ROSE) can be incorporated into cytology procedures. Our study aims to determine the efficacy of ROSE on the specimen adequacy of FNAC as compared to non-ROSE cases.
Methods:
We conducted a prospective study that included consecutive FNAC cases from various sites between January 10th, 2025, and April 9th, 2025. All cases performed in our laboratory's FNAC clinic had undergone ROSE using a Diff-Quik stain, whereas all FNAC cases in which the glass slides were received from other institutions for cytological evaluation were not subjected to ROSE. The specimen adequacy of ROSE-performed FNAC cases was compared with that of the non-ROSE group.
Results:
This study included 350 patients. ROSE-performed cases constituted 30.8% (n = 108), while non-ROSE cases accounted for the remaining 69.2% (n = 242). The thyroid was the most frequently sampled site overall (38.86%, n = 136), followed by lymph nodes (31.14%, n = 109), breast (16.58%, n = 58), salivary gland (9.14%, n = 32), and soft tissue (3.14%, n = 11). Cell block preparations were performed in 22.85% (n = 80) of cases, of which 88.75% (n = 71) belonged to the ROSE group. Among the 286 overall adequate cases (81.7%), ROSE was performed in 106 cases (98.14% specimen adequacy, p < 0.001), while 180 out of 242 cases in the non-ROSE group were adequate (74.38% specimen adequacy, p < 0.001).
Conclusion:
The current study validates ROSE as a relevant and beneficial method that can be used as an adjunct to FNAC to improve the specimen adequacy.
