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Related Experiment Video

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Rapid Phenotypic Antimicrobial Susceptibility Testing with Multichannel Large-Volume Scattering Imaging and a

Jiapei Jiang1, Shuang Zhou2, Michelle McBride1

  • 1Biodesign Center for Bioelectronics and Biosensors, Arizona State University, Tempe, Arizona 85287, United States.

Analytical Chemistry
|December 1, 2025
PubMed
Summary
This summary is machine-generated.

Rapid antimicrobial susceptibility testing (AST) is crucial for treating multidrug-resistant (MDR) infections. A new Large-Volume Scattering imaging (LVSim) system provides precise phenotypic AST and minimum inhibitory concentration (MIC) results in under 2 hours.

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Area of Science:

  • Microbiology
  • Biomedical Engineering
  • Infectious Diseases

Background:

  • Multidrug-resistant (MDR) bacterial infections pose a significant global health threat, especially in critical conditions like septic shock.
  • Delays in identifying effective antibiotic treatments for MDR infections directly correlate with increased patient mortality.
  • Current antimicrobial susceptibility testing (AST) methods can be time-consuming, hindering timely clinical decision-making.

Purpose of the Study:

  • To develop and validate a novel multichannel Large-Volume Scattering imaging (LVSim) system for rapid and precise phenotypic AST.
  • To enable simultaneous monitoring of multiple sample/drug conditions for efficient testing.
  • To achieve rapid determination of minimum inhibitory concentration (MIC) for guiding antibiotic therapy.

Main Methods:

  • Development of a multichannel Large-Volume Scattering imaging (LVSim) system capable of monitoring up to eight conditions concurrently.
  • Application of a Bayesian Gaussian process model to analyze bacterial growth dynamics over time.
  • Validation using *Escherichia coli* and *Pseudomonas aeruginosa* (including a slow-growing MDR clinical isolate).

Main Results:

  • The LVSim system successfully achieved rapid MIC determination within 2 hours for tested bacterial strains.
  • The system demonstrated precision in phenotypic AST, distinguishing susceptibility profiles.
  • Simultaneous monitoring capability allows for efficient evaluation of multiple antibiotic treatments.

Conclusions:

  • The multiplexed LVSim system represents a significant advancement in rapid phenotypic AST.
  • This technology offers a promising solution for combating challenging multidrug-resistant bacterial infections.
  • Faster MIC determination using LVSim can lead to more timely and effective patient treatment, potentially reducing mortality.