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AMH Concentration and Gene Expression Level in Each Follicle and Their Relationship With Fertilization and Blastocyst
Asuka Takeda1, Yuri Yamamoto1, Shota Yamamoto1,2
1Department of Obstetrics and Gynecology, Graduate School of Biomedical Sciences Tokushima University Tokushima Japan.
Reproductive Medicine and Biology
|December 1, 2025
Summary
Follicular fluid Anti-Mullerian hormone (AMH) levels, but not granulosa cell AMH gene expression, may influence oocyte fertilization capacity. Further research is needed to understand AMH
Area of Science:
- Reproductive Endocrinology
- In Vitro Fertilization
- Ovarian Biology
Background:
- Anti-Mullerian hormone (AMH) plays a crucial role in ovarian follicle development.
- Understanding AMH dynamics within the follicle is essential for assessing oocyte quality and predicting reproductive outcomes.
- Previous studies have primarily focused on serum AMH levels, with less known about its local concentration and gene expression within the follicle.
Purpose of the Study:
- To investigate the relationship between Anti-Mullerian hormone (AMH) concentrations in follicular fluid (FF) and AMH gene expression in granulosa cells (GCs).
- To determine the association of FF AMH and GC AMH mRNA levels with oocyte maturity, fertilization, and blastocyst development.
- To explore the correlation between serum AMH and FF AMH concentrations.
Main Methods:
- Analysis of 270 follicular fluid (FF) samples and 140 granulosa cell (GC) samples from patients undergoing intracytoplasmic sperm injection (ICSI).
- Quantification of AMH concentrations in FF and AMH mRNA expression levels in GCs.
- Correlation analysis between AMH levels, oocyte characteristics, and embryological outcomes.
Main Results:
- Follicular fluid AMH concentrations and GC AMH mRNA expression varied significantly between follicles and showed no direct correlation with each other.
- Serum AMH concentrations positively correlated with mean FF AMH concentrations (r²=0.78, p<0.05).
- FF AMH concentrations were lower in fertilized oocytes compared to unfertilized oocytes, while GC AMH mRNA expression did not correlate with clinical outcomes.
Conclusions:
- Follicular fluid AMH concentrations may be linked to oocyte fertilization capacity.
- Granulosa cell AMH gene expression levels do not appear to be directly associated with embryological outcomes in ICSI.
- The study highlights the potential role of local FF AMH as a biomarker for oocyte fertilization potential.
Keywords:
anti‐mullerian hormoneclinical outcomesfollicular fluidgranulosa cellsintracytoplasmic sperm injectionMore Related Videos
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