Related Experiment Video

Updated: Jul 23, 2026

Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses
11:17

Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses

Published on: August 30, 2018

13.3K

Construction and validation of a risk assessment scale for multidrug-resistant bacteria infections in critically ill

Jia Zhao1, Li Yuan2, Yujing Wang1

  • 1School of Nursing, Xinjiang Medical University, Xinjiang, China.

Frontiers in Medicine
|December 1, 2025
PubMed
Abstract

Insights

A new risk assessment scale for multidrug-resistant organism (MDRO) infections in critically ill patients has been developed. This scale demonstrates good reliability and validity for assessing infection risk in intensive care units.

Area of Science:

  • Critical care medicine
  • Infectious diseases
  • Health informatics

Background:

  • Multidrug-resistant organisms (MDROs) pose a significant threat to patients in intensive care units (ICUs).
  • Accurate risk assessment for MDRO infections is crucial for timely intervention and improved patient outcomes in critical illness.

Purpose of the Study:

  • To develop and validate a reliable and scientifically sound risk assessment scale for MDRO infections.
  • To provide a tool for healthcare professionals to systematically evaluate infection risk in critically ill patients.

Main Methods:

  • A systematic literature review, Delphi method, and analytic hierarchy process were used to develop the initial scale.
  • Exploratory and confirmatory factor analyses were conducted on data from 750 critically ill patients in China.
  • Reliability was assessed using Cronbach's alpha, and validity was evaluated through content validity index and factor analysis.

Main Results:

  • The developed scale comprises five dimensions and 20 items, showing high internal consistency (Cronbach's alpha = 0.873) and content validity (CVI = 0.925).
  • Factor analysis confirmed the scale's structure, explaining 67.861% of the variance.
  • The scale demonstrated good discriminative capability with an AUC of 0.788, sensitivity of 0.741, and specificity of 0.707.

Conclusions:

  • The risk assessment scale for MDRO infection in critically ill patients exhibits excellent reliability and validity.
  • This scale offers a scientific and dependable method for assessing infection risk, aiding clinical decision-making.
Keywords:
Delphi methodmulti-drug resistant bacteriareliabilityscalevalidity

More Related Videos

Tools for the Real-Time Assessment of a Pseudomonas aeruginosa Infection Model07:39

Tools for the Real-Time Assessment of a Pseudomonas aeruginosa Infection Model

Published on: April 6, 2021

3.9K
Author Spotlight: Accelerating Diagnostic Accuracy with Direct Identification of Gram-Negatives from Blood Culture Bottles09:07

Author Spotlight: Accelerating Diagnostic Accuracy with Direct Identification of Gram-Negatives from Blood Culture Bottles

Published on: May 24, 2024

1.4K

Related Concept Videos

Factors Affecting the Risk of Infection01:26

Factors Affecting the Risk of Infection

The hosts' susceptibility to infection depends on several factors. The integrity of the skin and mucous membranes helps protect the body against microbial attacks. When the skin is altered, the chance of infection, limb loss, and even death increases.
The integrity and count of the white blood cells help the body resist pathogens and fight infection. When impaired, it reduces the body's resistance to pathogens. The acidic pH levels of the gastrointestinal, genitourinary tracts, and skin create...
Healthcare Associated Infections II: Preventive Measures01:22

Healthcare Associated Infections II: Preventive Measures

Essential infection prevention measures are based on the knowledge of the infection chain, the modes of transmission in healthcare settings, and the use of the best practices in all healthcare settings. Compulsory public reporting of healthcare-associated infection rates is needed to allow individuals and the community to make informed choices regarding selecting a healthcare facility.
The best practices for preventing healthcare-associated infections include hand hygiene, patient risk...
Pneumonia III: Complications and Assessment01:30

Pneumonia III: Complications and Assessment

Pneumonia poses the potential for numerous complications that warrant consideration. These complications include the following:
Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches01:23

Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches

Biopharmaceutical studies constitute a vital field aiming to enhance drug delivery methods and refine therapeutic approaches, drawing upon diverse interdisciplinary knowledge. In research methodologies, the choice between controlled and non-controlled studies significantly influences the study's reliability and accuracy.
Non-controlled studies, commonly employed for initial exploration, lack a control group, rendering them susceptible to biases and external influences. In contrast, controlled...
Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...