Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

1.7K
In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
1.7K
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

1.2K
Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
1.2K
Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle01:19

Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle

1.6K
Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...
1.6K
Mass Spectrometry: Isotope Effect01:13

Mass Spectrometry: Isotope Effect

3.8K
Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the mass differences between isotopes. Furthermore, the intensity of these signals is dependent on the...
3.8K
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

620
Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....
620
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

2.3K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Standard in the rinse.

Talanta·2025
Same author

Multichannel Dilution Analysis Using a Single Peristaltic Pump Tube.

Applied spectroscopy·2025
Same author

Calibration by Proxy.

Analytical chemistry·2024
Same author

Detecting Temporal Changes of Self-Absorption in a Laser-Induced Copper Plasma from Time-Resolved Photomultiplier Signal Emission Profiles.

Applied spectroscopy·2018
Same journal

Impact of an Artificial Albumin Corona on Surface Charge-Driven Nano-Bio Interactions and Cytotoxicity of Silver Nanoparticles.

ACS omega·2026
Same journal

Structural and Functional Disruption of Thiopurine S‑Methyltransferase by the A80P Variant: A Simulation and Genotyping Study.

ACS omega·2026
Same journal

CRISPR/Cas12a2-Mediated Ultrasensitive Assay for Rapid Detection of H1N1 Influenza Virus RNA.

ACS omega·2026
Same journal

Photocatalytic Treatment of Real Sugar Industry Wastewater Using Lignocellulosic Biomass-Derived Hydrochar/g-CN.

ACS omega·2026
Same journal

Electrochemical Dopamine Biosensor Based on Plant-Derived Peroxidase Immobilized on Titanate Nanowires.

ACS omega·2026
Same journal

Revealing the Effects of Process Parameters on Structural, Thermal, Mechanical, Biodegradation, and Biocompatibility Properties on the Electrospinning of Poly(vinyl alcohol)/Microbial Inulin Nanofibers.

ACS omega·2026
See all related articles

Related Experiment Video

Updated: Jan 9, 2026

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

4.8K

Multi-Isotope Internal Standardization for Inductively Coupled Plasma Mass Spectrometry.

Micah X DeCourcey1, Abigail J Crossman1, Willis B Jones1

  • 1Department of Chemistry and Biochemistry, University of North Florida, Jacksonville, Florida 32224, USA.

ACS Omega
|December 1, 2025
PubMed
Summary
This summary is machine-generated.

Multi-isotope internal standardization (MIIS) is a novel calibration method for inductively coupled plasma mass spectrometry (ICP-MS). This technique enhances accuracy and efficiency in trace analyte determination, outperforming traditional methods.

More Related Videos

A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer SMPS-ICPMS
11:18

A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer SMPS-ICPMS

Published on: July 11, 2017

11.1K
Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
09:24

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: December 18, 2020

6.0K

Related Experiment Videos

Last Updated: Jan 9, 2026

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

4.8K
A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer SMPS-ICPMS
11:18

A Practical Guide on Coupling a Scanning Mobility Sizer and Inductively Coupled Plasma Mass Spectrometer SMPS-ICPMS

Published on: July 11, 2017

11.1K
Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
09:24

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: December 18, 2020

6.0K

Area of Science:

  • Analytical Chemistry
  • Spectrometry

Background:

  • Traditional trace analyte determination relies on single signal-to-concentration relationships.
  • Existing multisignal methods face limitations like low throughput, poor accuracy in complex matrices, and requirement for multiple signals per analyte.

Purpose of the Study:

  • To introduce and evaluate Multi-Isotope Internal Standardization (MIIS) as an advanced calibration method for ICP-MS.
  • To address limitations of existing multisignal techniques in trace analyte determination.

Main Methods:

  • Developed a novel calibration method, MIIS, utilizing multiple isotope masses for analytes and internal standards.
  • Employed only two calibration solutions for constructing the calibration curve.
  • Validated MIIS through spike recovery experiments in complex matrices and analysis of certified reference materials.

Main Results:

  • MIIS achieved approximately 100% analyte recovery with low relative standard deviations (few percent) in challenging sample matrices.
  • Demonstrated superior performance compared to traditional calibration and other multisignal methods.
  • Validation in certified reference materials showed recoveries ranging from 85% to 111%.

Conclusions:

  • MIIS offers a significant advancement in ICP-MS calibration for trace analyte determination.
  • The method provides a high number of calibration points with minimal solution preparation.
  • MIIS overcomes limitations of previous techniques, enhancing accuracy and efficiency.