Snap bean diversity for resistance to bean common mosaic and necrosis viruses examined using new and existing...

Snap bean (Phaseolus vulgaris L.), a nutrient-rich crop, is limited by Bean common mosaic virus (BCMV) and Bean common mosaic necrosis virus (BCMNV). To better understand the genetic basis of resistance, 376 snap bean accessions and 12 F₂ populations were screened with US-6 [pathogroup (PG)-VII], NL-8 (PG-III), or NL-3 (PG-VI) strains and genotyped for I, bc-1, and bc-3 loci using existing candidate gene-linked markers. Two Vps4 candidate genes associated with bc-u and bc-2 recessive loci were re-sequenced to identify novel mutations. Two major allelic groups of bc-u missense mutations were identified: (bc-u ^a = bc-u ^d = bc-u ^g) and (bc-u ^r ≤ bc-u ^s), each interacting differently with bc-1 and bc-2 to confer strain-specific resistance or susceptibility to NL-3 and US-6. New bc-2 ^{[IVT 7214]} and existing bc-2 ^[Robust] and bc-2 ^[UI-111] nonsense mutations exhibited functionally equivalent alleles, resulting in the same genetic effect across pathogroups. Twenty-six allelic combinations were found, 16 of which included the dominant I gene. Overall, 336 of 376 accessions carried I, of which 233 possessed I + RNase H-Like 1 ^C + bc-1 combination characteristic of host group (HG)-9 cultivars with restricted vein necrosis (VN) to NL-8. Importantly, 30 accessions possessed I + RNase H-Like 1 ^C + bc-1 + bc-u ^{a, d, or g}, conferring VN to NL-3 strain, characteristic of HG-10 cultivars. This panel provides a valuable resource of resistance diversity to BCMV and BCMNV, which can be used in breeding programs with the markers described. The bc-2 and bc-3 alleles, found in only one or two accessions, offer untapped resistance sources for snap bean.

The online version contains supplementary material available at 10.1007/s11032-025-01605-w.