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Extraction and Purification of Polyphenols from Freeze-dried Berry Powder for the Treatment of Vascular Smooth Muscle Cells In Vitro
Published on: July 5, 2017
Advanced recovery of bioactive polyphenols: integrated optimization of drying and green extraction for avocado seed
Jorge G Figueroa1,2, Isabel Borrás-Linares1, Jesús Lozano-Sánchez3
1Department of Analytical Chemistry, University of Granada, Avda. Fuente Nueva s/n, 18071 Granada, Spain.
Abstract:
Response surface methodology using a central composite design was applied to optimize the accelerated solvent extraction (ASE) of phenolic compounds from avocado seed and seed coat by-products. The optimal extraction conditions for both matrices were achieved at 200 °C using as extractant solvent a mixture of ethanol:water (50,50, v/v). Additionally, the drying process was optimized for avocado seed samples at different temperatures (45, 55, 65, 75, and 85 °C). Changes in the phenolic profiles during the drying and extraction processed were monitorized using HPLC-TOF-MS. A total of 42 and 52 phenolic compounds were individually identified and quantified in avocado seed and seed coat, respectively. The optimal drying temperature for avocado seed was 85 °C. In contrast, the seed coat exhibited different optimal temperatures depending on the phenolic group under research, with 75 °C being the most favourable for the majority of compounds evaluated.
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