Calculation of specificity constants Γ for RUBISCO of different species from microcalorimetric data
1, Morsestrasse 11, 10587, Berlin, Germany.
Abstract:
Based on earlier data concerning the reaction enthalpy [Formula: see text] of Ribulose 1,5-bisphosphate carboxylase/oxygenase (RUBISCO) from spinach (Frank et al. Phys Chem Chem Phys 2:1301-1304, 2000), it is shown that the specificity constant [Formula: see text], indicating the ability of RUBISCO to discriminate between CO2 and O2 as substrate, can be determined from an isothermal titration calorimetric (ITC) measurement of [Formula: see text] as a function of the substrate concentration ratio [Formula: see text]. The approach does not need any radioactive materials and might be a cost-effective alternative for RUBISCO engineering to screen for variants with a high specificity constant.
Related Concept Videos
01:46The Calvin Benson Cycle
02:46Calculating the Equilibrium Constant
For example, gaseous nitrogen dioxide forms dinitrogen tetroxide according to this equation:
01:20Determination of Michaelis Constant and Maximum Elimination Rate
These parameters can be estimated by analyzing plasma concentration data post-drug administration. A notable example of this application is phenytoin, a drug with capacity-limited kinetics. It's recommended that phenytoin should be administered at two...