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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Updated: Jul 23, 2026

Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging

Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging

Published on: April 2, 2008

Conjugated Polymer Nanoparticles for Fluorescence Imaging in Stem Cell Tracking.

Merve Beyaz¹, Başak Çoban², Fatih Sema¹

¹Department of Chemistry, Izmir Institute of Technology (IZTECH), Izmir, Turkey.

Methods in Molecular Biology (Clifton, N.J.)

|December 1, 2025

Summary

Conjugated polymer nanoparticles (CPNs) offer advanced fluorescent probes for stem cell imaging and tracking in regenerative medicine. Their unique properties enable long-term cell labeling and monitoring, paving the way for next-generation therapies.

Keywords:

Conjugated polymer nanoparticles Fluorescence NIR imaging Optical imaging Regenerative medicine Stem cell tracking

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Last Updated: Jul 23, 2026

Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging

Labeling Stem Cells with Fluorescent Dyes for non-invasive Detection with Optical Imaging

Published on: April 2, 2008

Harmonic Nanoparticles for Regenerative Research

Harmonic Nanoparticles for Regenerative Research

Published on: May 1, 2014

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

Published on: August 6, 2014

Area of Science:

Biomedical Engineering
Materials Science
Regenerative Medicine

Background:

Optical imaging is crucial for stem cell tracking in regenerative medicine due to its sensitivity and accessibility.
Conjugated polymer nanoparticles (CPNs) are emerging as superior fluorescent probes, offering high photostability and tunable properties.

Purpose of the Study:

To provide a comprehensive overview of CPNs for stem cell imaging.
To discuss CPNs' properties, labeling strategies, and performance in optical imaging.
To highlight applications and challenges of CPNs in regenerative medicine.

Main Methods:

Review of literature on CPNs for stem cell imaging.
Analysis of CPN structural and photophysical properties.
Evaluation of cellular labeling techniques and imaging performance.

Main Results:

CPNs demonstrate versatility for long-term stem cell labeling and noninvasive monitoring.
Applications include tracking cell migration, proliferation, and differentiation.
Challenges include cytotoxicity, biodegradability, and reproducibility.

Conclusions:

CPNs hold significant potential for advancing stem cell tracking tools.
Emerging approaches like NIR-II probes and stimuli-responsive designs show promise.
CPNs are key to developing next-generation regenerative medicine therapies.