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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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Related Experiment Video

Updated: Jan 9, 2026

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology
09:45

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology

Published on: November 14, 2025

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Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology.

Zheyu Liu1, Ye Tian2, Zian Zhang1

  • 1Department of Urology, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine.

Journal of Visualized Experiments : Jove
|December 1, 2025
PubMed
Summary

This study introduces an integrated microfluidic method for analyzing circulating tumor cells (CTCs). The new protocol enhances CTC capture, purity, and single-cell sequencing for improved cancer diagnosis and treatment monitoring.

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Last Updated: Jan 9, 2026

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology
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Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology

Published on: November 14, 2025

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Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells
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Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment
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Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment

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Area of Science:

  • Oncology
  • Biotechnology
  • Genomics

Background:

  • Circulating tumor cells (CTCs) are vital biomarkers for monitoring cancer metastasis and progression.
  • Current bulk CTC analyses lack the resolution to capture cellular heterogeneity, limiting insights into tumor biology.
  • Existing single-cell sequencing methods for CTCs face challenges with low recovery, labor intensity, and contamination.

Purpose of the Study:

  • To develop an integrated microfluidic workflow for efficient CTC enrichment, purification, and single-cell sequencing.
  • To overcome the limitations of current single-CTC analysis techniques, improving throughput and reducing costs.
  • To enable high-resolution characterization of CTC heterogeneity for precision oncology.

Main Methods:

  • An integrated microfluidic protocol combining CTC enrichment, purification, and single-cell sequencing.
  • Dynamic magnetic capture in a herringbone chip for robust CTC isolation.
  • Leukocyte antibody-coated chip for negative selection-based purification.
  • Differential flow resistance chip for high-precision single-cell capture and barcoding.

Main Results:

  • Significantly enhanced CTC capture efficiency and purity.
  • Improved single-cell sequencing throughput and reduced costs compared to Poisson distribution-based methods.
  • Demonstrated a unified workflow for CTC analysis from enrichment to sequencing.

Conclusions:

  • The developed integrated microfluidic protocol offers a more precise and scalable method for analyzing CTC heterogeneity.
  • This approach is suitable for clinical applications and large-scale cancer research, advancing precision oncology.
  • Potential to refine early cancer diagnosis, treatment monitoring, and metastasis research.