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Published on: November 9, 2018
Rapid Isolation of Human Breast Milk-Derived Extracellular Vesicles
Jarren R Oates1, Ayodeji Ipinmoroti2, Colin Martin3
1Department of Pediatrics/Division of Neonatology and Center of Glial Biology in Medicine, University of Alabama at Birmingham School of Medicine; School of Health Professions/Department of Clinical and Diagnostic Sciences, University of Alabama at Birmingham.
A new method isolates functional human breast milk extracellular vesicles (HBMDEVs) quickly and efficiently. This advance could enable rapid therapeutic use of HBMDEVs for conditions like necrotizing enterocolitis in premature infants.
Area of Science:
- Biomedical Engineering
- Neonatal Research
- Nanotechnology
Background:
- Extracellular vesicles (EVs) are nanoparticles with therapeutic potential.
- Human breast milk-derived extracellular vesicles (HBMDEVs) show promise for treating necrotizing enterocolitis in premature infants.
- Current EV isolation methods are impractical for clinical settings due to time and equipment constraints.
Purpose of the Study:
- To develop a simple, rapid isolation method for HBMDEVs suitable for clinical application.
- To enable timely therapeutic administration of HBMDEVs.
Main Methods:
- A novel technique combining centrifugation and ultrafiltration of human breast milk.
- Centrifugation removes fat and cellular debris.
- Ultrafiltration isolates high-yield, functional HBMDEVs from skim milk.
Main Results:
- The proposed method yields functional extracellular vesicles.
- This technique is faster and more practical than traditional isolation methods.
- It offers a high yield of HBMDEVs.
Conclusions:
- This new isolation method provides a practical approach for obtaining HBMDEVs.
- It facilitates the potential clinical use of HBMDEVs as a therapeutic agent.
- This method could improve treatment outcomes for premature infants with necrotizing enterocolitis.
