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Biosensors for coenzyme A thioester derivatives: Development, optimization and applications.

Jing Qiu1, Amna Bibi2, Alvaro R Lara3

  • 1Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China; Sino-Danish College, University of Chinese Academy of Sciences, Beijing 101408, China; National Center of Technology Innovation for Synthetic Biology, Tianjin 300308, China; Sino-Danish Centre for Education and Research, Beijing 101408, China.

Biotechnology Advances
|December 1, 2025
PubMed
Summary
This summary is machine-generated.

Biosensors enable real-time monitoring of essential microbial metabolites like acetyl-CoA and malonyl-CoA. This review details biosensor applications, limitations, and future prospects for metabolic engineering.

Keywords:
BiosensorsCoA thioester derivativesDynamic regulationMetabolic engineeringReal-time monitoringSynthetic biology

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Area of Science:

  • Biochemistry and Molecular Biology
  • Metabolic Engineering
  • Microbiology

Background:

  • Coenzyme A (CoA) thioester derivatives, including acetyl-CoA, malonyl-CoA, and fatty acyl-CoA, are critical central metabolites in microorganisms.
  • These metabolites are vital for numerous metabolic pathways and serve as precursors for synthesizing high-value compounds like fatty acids, polyketides, and flavonoids.
  • The dynamic and tightly regulated spatiotemporal distribution of CoA thioesters necessitates real-time monitoring.

Purpose of the Study:

  • To provide a comprehensive review of biosensor development for CoA thioester derivatives.
  • To discuss the progress, optimization, applications, and limitations of these biosensors.
  • To outline future prospects and potential biosensor elements for CoA thioester derivatives.

Main Methods:

  • Literature review of existing biosensor technologies for CoA thioesters.
  • Analysis of biosensor performance, including sensitivity, specificity, and dynamic range.
  • Evaluation of applications in metabolic engineering, such as dynamic regulation and high-throughput screening.

Main Results:

  • Biosensors offer rapid and immediate detection of CoA thioester derivatives in response to inducers.
  • These tools are instrumental in advancing metabolic engineering strategies for microbial systems.
  • Current biosensors have specific limitations that require further development.

Conclusions:

  • Biosensors are powerful tools for understanding and manipulating microbial metabolism related to CoA thioesters.
  • Addressing current limitations will enhance the utility of biosensors for metabolic engineering.
  • Further research into novel biosensor elements holds promise for improved detection and regulation.