A review of transcriptional control and adaptive functions in terpenoid biosynthesis: Focus on MYB regulatory...

Terpenoids constitute one of the most chemically diverse and functionally significant classes of plant secondary metabolites, involved in growth regulation, environmental responsiveness, and interactions with biotic factors. In fruit-bearing plants, terpenoids not only contribute to aromatic traits that affect flavor and commercial value but also serve as natural agents of defense. The biosynthesis of terpenoids proceeds through two evolutionarily conserved metabolic routes: the cytosolic mevalonate (MVA) pathway and the plastid-localized methylerythritol phosphate (MEP) pathway. At the final stage of these pathways, terpene synthases (TPSs) catalyze the formation of structurally diverse terpenoid compounds and are considered key enzymes in terpenoid biosynthesis. Recent genomic and functional studies have revealed both the expansion and diversification of TPS gene families across various horticultural species and the increasing complexity of their transcriptional regulation. This has led to growing interest in the transcriptional regulatory networks that coordinate terpenoid metabolism. Among these regulators, MYB transcription factors have emerged as central components, which can directly activate or repress the expression of TPS and other pathway genes and often function through cooperative or antagonistic interactions with other transcription factor families. Additionally, MYB factors respond to various environmental and endogenous signals such as light, hormones, and nutrient availability, positioning them as crucial regulators in adaptive terpenoid metabolic control.