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Related Experiment Video

Updated: Jan 9, 2026

Expansion Microscopy: High-Resolution Fluorescent Imaging with a Conventional Microscope

Expansion Microscopy: High-Resolution Fluorescent Imaging with a Conventional Microscope

Published on: December 19, 2025

Expansion omics: from expansion microscopy to spatial omics.

Zhen Dong^1,2,3, Weirong Xiang^4,5,6, Wenhao Jiang^4,5,6

¹Affiliated Hangzhou First People's Hospital, State Key Laboratory of Medical Proteomics, School of Medicine, Westlake University, Hangzhou, Zhejiang Province, China. dongzhen@westlake.edu.cn.

Molecular Systems Biology

|December 1, 2025

Summary

This summary is machine-generated.

Expansion omics (ExO) uses physical tissue expansion for high-resolution spatial omics profiling. This technology enables precise biomolecule detection and mapping across multiple omics layers, advancing spatial biology and medicine.

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Area of Science:

Spatial biology
Molecular imaging
Omics technologies

Background:

Tissue expansion (ExO) is a technique initially for super-resolution imaging.
ExO has evolved into a foundational method for spatially resolved omics profiling.
It enables precise biomolecule detection and links them to biological functions.

Purpose of the Study:

To explore how expansion omics integrates multi-omics data.
To highlight the role of chemical anchoring strategies in ExO.
To discuss the advancements in spatial resolution achieved by ExO.

Main Methods:

Utilizing physical tissue expansion for enhanced spatial resolution.
Employing chemical anchoring strategies for selective molecular retention.
Applying ExO across diverse spatial omics modalities like epigenomics, transcriptomics, proteomics, and lipidomics.

Main Results:

Achieved subcellular resolution for omics profiling and sub-nanometer scale for imaging.
Enabled high-resolution mapping of chromatin states, gene expression, protein localization, and lipid distributions.
Facilitated spatial multi-omics approaches for correlating multiple biomolecular dimensions.

Conclusions:

Expansion omics is a core technology for whole-slide, single-cell multi-omics.
ExO integrates super-resolution imaging with spatial omics for advanced biological insights.
Further development is needed to address challenges in resolution, molecular retention, and data analysis for the Artificial Intelligence Virtual Cell.