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Updated: Jan 9, 2026
Innovative Strategies for Organ Preservation in Heart Transplantation: Uniform Cooling Preservation and Ex-situ Normothermic Perfusion
Published on: November 28, 2025
A New Colloid Preservation Solution for Ex Situ Hypothermic Perfusion of the Heart: A Preclinical Pilot Study
Simon Dang Van1,2,3,4, Dorothée Brunet2,5, Benoit Decante2
1Department of Cardiac Surgery, University Hospital of Angers, Angers, France.
Background:
Hypothermic ex situ heart graft perfusion using acellular preservation solution is a promising technique for heart transplantation.
Methods:
We investigated the cardioprotective effects of a novel preservation solution, IGL-2 (Institut Georges Lopez, France), in a porcine model of hypothermic ex situ heart perfusion over 4 h, following either beating-heart procurement or donation after circulatory death.
Results:
Coronary endothelial injury (assessed by Endothelin-1) and oxidative stress markers (assessed by nitrates and hydrogen peroxide) were evaluated through repeated perfusate sampling. Cardiac power output was measured after ex situ warm reperfusion in working mode.
Conclusion:
Preservation with IGL-2 was associated with reduced endothelial injury and oxidative stress, resulting in improved myocardial recovery following blood reperfusion.
