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Intra-Rater Variability and Inter-Rater Reliability in Measuring Tear Osmolarity in Dogs Using the ScoutPro System.

Joschka Spornberger¹, Petr Soukup¹, Andrea Meyer-Lindenberg²

¹Animal Eye Practice, Berlin, Germany.

Veterinary Ophthalmology

|December 2, 2025

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The ScoutPro osmolarity system offers a straightforward method for measuring tear film osmolarity in dogs. This study found the system

Area of Science:

Veterinary Ophthalmology
Ocular Surface Disease
Diagnostic Technology

Background:

Tear film osmolarity is a key indicator of ocular surface health.
Accurate tear osmolarity measurement is crucial for diagnosing and managing dry eye disease in canines.
The ScoutPro system presents a novel tool for assessing tear osmolarity.

Purpose of the Study:

To assess the applicability and reliability of the ScoutPro osmolarity system for measuring tear film osmolarity in healthy dogs.
To evaluate the ease of use and reproducibility of the ScoutPro system by multiple examiners.

Main Methods:

Seventeen healthy dogs of various breeds were enrolled.
Tear osmolarity was measured using the ScoutPro osmolarity system by two examiners.

Keywords:

I‐PEN Vet TearLab canine dry eye disease ocular surface tear film

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Intra-rater variability and inter-rater reliability were calculated through repeated measurements.

Main Results:

The ScoutPro system demonstrated ease of use and straightforward handling.
Mean tear osmolarity values were similar between the two examiners (323.51 ± 9.89 mOsm/L and 326.74 ± 9.02 mOsm/L).
Acceptable intra-rater variability (Cronbach's Alpha 0.75-0.80) and no significant inter-rater differences were observed.

Conclusions:

The ScoutPro osmolarity system is applicable and easy to use for tear film osmolarity measurement in healthy dogs.
The system shows acceptable variability and inter-rater reliability in this cohort.
Further research is recommended to validate these findings and explore its use in dogs with ocular surface disease.