Effect of Saliva on Compressive Strength of Mineral Trioxide Aggregate, Biod+, and Biodentine: An In Vitro Study

The study aimed to evaluate the effect of saliva on the compressive strength of three commonly used dental materials-mineral trioxide aggregate (MTA), Biod+, and Biodentine-used for root canal fillings.

Thirty-six extracted primary molars were divided into two groups: a control group (group A) with no saliva exposure and an experimental group (group B) subjected to saliva exposure. Each group was further divided into three subgroups (n = 6), with specimens filled with MTA, Biod+, or Biodentine. After a standardized pulpotomy procedure, the materials were placed within the prepared cavities. The compressive strength of each material was tested using an Instron universal testing machine after a 4-hour setting period. Data were analyzed using one-way analysis of variance (ANOVA).

Biod+ demonstrated the highest compressive strength among the three materials, both in the presence and absence of saliva. Biodentine exhibited the least variation in compressive strength following saliva exposure, indicating superior stability in an oral environment. MTA showed lower compressive strength compared to Biod+ and Biodentine. Statistical analysis confirmed a significant difference in compressive strength between the groups (p = 0.00532).

Biod+ exhibited the highest durability in terms of compressive strength, making it a promising material for endodontic applications requiring high mechanical resistance. Biodentine, on the other hand, demonstrated greater stability under salivary exposure, making it a reliable choice for clinical scenarios where moisture contamination is a concern. These findings provide valuable insights into the selection of optimal root canal filling materials based on specific clinical requirements.

Bhatnagar S, Jeevanandan G. Effect of Saliva on Compressive Strength of Mineral Trioxide Aggregate, Biod+, and Biodentine: An In Vitro Study. Int J Clin Pediatr Dent 2025;18(11):1345-1348.