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Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast
Published on: October 23, 2016
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Clathrin-mediated endocytosis in budding yeast at a glance: animated
Leanna Owen1, Margot Riggi2, David G Drubin1
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720-3202, USA.
Journal of Cell Science
|December 2, 2025
Summary
Clathrin-mediated endocytosis (CME) is vital for cells. This study visualizes CME in yeast, detailing protein dynamics and actin networks driving membrane internalization.
Area of Science:
- Cell Biology
- Molecular Biology
- Biophysics
Background:
- Clathrin-mediated endocytosis (CME) is a fundamental cellular process for internalizing molecules.
- CME involves intricate protein assembly and disassembly at the plasma membrane.
- Budding yeast is a key model organism for studying CME.
Purpose of the Study:
- To create a comprehensive 3D molecular animation of CME progression in budding yeast.
- To visualize the spatial and temporal dynamics of proteins involved in CME.
- To highlight recent findings on protein condensation and actin-driven membrane deformation.
Main Methods:
- Integration of decades of quantitative genetic, biochemical, and imaging data.
- Development of a 3D molecular animation depicting CME.
- Analysis of protein condensation and actin network organization.
Main Results:
- A detailed 3D animation illustrating the complete CME pathway in budding yeast.
- Visualization of the dynamic interactions and localization of key CME proteins.
- Insights into the role of actin networks in overcoming turgor pressure during internalization.
Conclusions:
- The study provides a dynamic molecular view of CME in budding yeast.
- Understanding protein condensation and actin forces is crucial for CME.
- This work enhances the visualization and comprehension of this essential cellular process.
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