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Alternative RNA Splicing02:18

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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
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circASbase：A Comprehensive Database of Alternative Splicing Events in circRNAs.

Lingxiao Zou1, Jian Zhao1, Haojie Li2,3,4

  • 1Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 211106, China.

Genomics, Proteomics & Bioinformatics
|December 2, 2025
PubMed
Summary
This summary is machine-generated.

A new database, circASbase, catalogs over 450,000 circular RNA (circRNA) alternative splicing events across 13 species. This resource reveals unique splicing patterns in circRNAs, impacting their function and regulation.

Keywords:
Alternative splicingCircular RNACoding potentialDatabaseFull-length isoform

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Area of Science:

  • Molecular Biology
  • Bioinformatics
  • Genomics

Background:

  • Alternative splicing generates diverse RNA isoforms, including circular RNAs (circRNAs).
  • A lack of specialized databases hinders the study of circRNA alternative splicing events.
  • Understanding circRNA splicing is crucial for their functional diversity.

Purpose of the Study:

  • To develop circASbase, a comprehensive database for circRNA alternative splicing events.
  • To provide rich annotations for studying circRNA splicing regulation.
  • To facilitate research into the functional roles of circRNA alternative splicing.

Main Methods:

  • Cataloging 452,129 alternative splicing events in 884,047 full-length circRNAs.
  • Analyzing data from 581 samples across 13 species.
  • Developing user-friendly interfaces and web-based visualization tools.

Main Results:

  • Identified substantial differences in alternative splicing between circRNAs and linear transcripts.
  • Demonstrated that circRNA splicing events affect IRES sites, m6A sites, ORFs, and miRNA targets.
  • Showcased unique regulatory landscapes and functional impacts of circRNA splicing.

Conclusions:

  • circASbase addresses the need for circRNA splicing data repositories.
  • The database advances understanding of circRNA biology and regulatory mechanisms.
  • circASbase is a valuable resource for exploring circRNA alternative splicing and function.