LUMINIDEPENDENS orchestrates global transcriptional repression in Arabidopsis
Clara Bergis-Ser1, Qingyi Wang1, Xiaoning He1
1Université Paris-Saclay, CNRS, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Univ Evry, Institute of Plant Sciences Paris-Saclay, Orsay 91405, France.
Summary
LUMINIDEPENDENS (LD) is identified as a key factor in plant genome protection, suppressing widespread transcription and resolving replication stress. This discovery reveals a novel plant strategy for maintaining genomic integrity.
Area of Science:
- Plant molecular biology
- Genomics
- Cellular stress response
Background:
- Genomic integrity is crucial but threatened by transcription/replication conflicts, leading to replication stress.
- Mechanisms for resolving these conflicts are well-studied in animals, yeast, and prokaryotes, but less so in plants.
Purpose of the Study:
- To identify novel factors involved in mitigating replication stress in plants.
- To elucidate the role of LUMINIDEPENDENS (LD) in genome protection and transcription regulation.
Main Methods:
- Forward genetic screen to identify key factors.
- Transcriptomic analyses to assess gene expression changes.
- Chromatin immunoprecipitation and protein interaction studies to determine molecular mechanisms.
Main Results:
- LUMINIDEPENDENS (LD) was identified as a crucial factor for mitigating replication stress in Arabidopsis.
- LD acts as a global transcriptional repressor, downregulating over half of Arabidopsis genes upon its loss.
- LD directly binds the genome and interacts with the MED18 subunit of the Mediator complex, modulating RNA polymerase II phosphorylation.
Conclusions:
- LD plays a fundamental role in fine-tuning transcription genome-wide in plants.
- LD may suppress transcription-replication conflicts by dampening transcription and promoting replication fork progression.
- This study uncovers a unique genome-protective strategy in plants, offering insights into eukaryotic transcription-replication conflict management.
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