An Anti-Inflammatory and Pro-Angiogenic Porous Aloe Polysaccharide-Collagen Dressing Accelerates Wound Healing

Area of Science:

• Biomaterials Science
• Wound Healing Research
• Tissue Engineering

Background:

• Effective wound dressings are crucial for optimal healing outcomes.
• Developing advanced materials with enhanced properties is essential for managing complex wounds.
• Aloe polysaccharide and collagen are biocompatible materials with potential therapeutic benefits.

Purpose of the Study:

• To develop and characterize a novel porous composite wound dressing using aloe polysaccharide and collagen (AP-CD).
• To evaluate the physical properties, safety, and cytocompatibility of the AP-CD.
• To assess the in vivo efficacy of AP-CD in promoting wound healing.

Main Methods:

• Characterization of AP-CD using scanning electron microscopy and energy dispersive spectroscopy.
• Assessment of water absorption and air permeability.
• Evaluation of safety and cytocompatibility via CCK-8 and LDH assays.
• In vivo wound healing rate measurement, inflammatory response analysis, angiogenesis assessment, and single-cell sequencing.

Main Results:

• AP-CD exhibited favorable physical and chemical properties, along with good biocompatibility and safety.
• In vivo studies demonstrated that AP-CD significantly accelerated wound healing.
• AP-CD effectively suppressed inflammation, promoted angiogenesis, modulated growth and inflammatory factors, and altered immune cell populations (increased macrophages and T lymphocytes, decreased fibroblasts).

Conclusions:

• The developed AP-CD is a safe and effective wound dressing with superior properties.
• AP-CD promotes wound healing by inhibiting inflammation and enhancing angiogenesis.
• This novel composite dressing shows significant potential for clinical application in wound management.