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Dynamic Organellar Mapping in yeast reveals extensive protein localization changes during ER stress
Anna Platzek1, Klára Odehnalová1, Julia P Schessner2
1Heidelberg University Biochemistry Center, Heidelberg, Germany.
Nature Communications
|December 2, 2025
Summary
Investigating yeast cell biology, this study reveals widespread protein relocalization during endoplasmic reticulum (ER) stress using Dynamic Organellar Mapping. This technique uncovers major cellular reorganization and ER retention mechanisms.
Area of Science:
- Yeast cell biology
- Proteomics
- Cellular stress response
Background:
- Systematic studies in yeast cell biology are advanced.
- Assessing proteome-wide protein subcellular localization changes is challenging.
Purpose of the Study:
- To detect native, untagged protein localization changes during endoplasmic reticulum (ER) stress on a proteome-wide scale.
- To establish Dynamic Organellar Maps as a discovery tool for yeast cell biology.
Main Methods:
- Dynamic Organellar Mapping by label-free mass spectrometry.
- Analysis of protein relocalization during ER stress in yeast.
Main Results:
- Hundreds of proteins shift between cellular compartments during ER stress.
- Secretory pathway proteins accumulate in the ER, indicating selective retention of misfolded proteins.
- Identified candidate cargo proteins of the ER reflux pathway, reticulon cluster constituents, and altered nuclear import.
Conclusions:
- Protein relocalization is a significant component of cellular reorganization during ER stress.
- Dynamic Organellar Mapping is a powerful tool for discovering protein localization changes in yeast.
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