Chromosome-level genome assembly for an edible protein microalgae Auxenochlorella pyrenoidosa

The green algae Auxenochlorella pyrenoidosa is capable of synthesizing substantial amounts of protein, containing all essential and non-essential amino acids necessary for sustainable human nutrition. A. pyrenoidosa RLXCh3 is a novel germplasm isolated from soil, with 63.65% protein content. To achieve biofortification based on RLXCh3, PacBio II/IIe sequencing, alongside Hi-C sequencing and full-length transcriptome analysis, were performed to construct an improved chromosome-level haplotypic genome assembly consisting of 12 chromosomes and a genome size of 53.07 Mbp. Scaffold N50 (4.94 Mbp) and contig N50 (1.74 Mbp) were obtained, with a total of 12,091 protein-coding genes predicted. In summary, we acquired excellent A. pyrenoidosa isolates and reported the first chromosome-scale map of A. pyrenoidosa, providing a valuable resource for in-depth commercial protein bioproduction and nutrient biofortification.