Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Sociodemographic and clinical determinants of general practice consultation frequency among regular patients in Australia: a national study.

BJGP open·2026

Same author

A compact visible-light spectrometric detector for capillary electrophoresis.

Journal of chromatography. A·2026

Same author

The draft genome sequence of <i>Kitasatospora</i> sp. HPM-01-4 isolated from Mentaos Pine Forest, heart of Borneo, Indonesia.

Microbiology resource announcements·2026

Same author

Optical Tweezers in Emulsion Research: Principles, Advances, and Prospects.

Langmuir : the ACS journal of surfaces and colloids·2026

Same author

Noninvasive genotyping and early disease dynamics demonstrate the efficacy of ibrutinib in combination with immunochemotherapy in patients with mantle cell lymphoma treated in the TRIANGLE trial.

Leukemia·2025

Same author

Correlation between P53 immunohistochemical staining and TP53 molecular testing in endometrial carcinomas: a detailed assessment of discrepant cases with implications for patient management.

Histopathology·2025

Same journal

Integrated multi-assessment and structural performance index framework for stacking-sequence optimisation of natural fibre reinforced laminates.

Scientific reports·2026

Same journal

SuperiorGAT: graph attention networks for sparse LiDAR point cloud reconstruction in autonomous systems.

Scientific reports·2026

Same journal

The effect of stretching the pectoralis major, sternocleidomastoid, and iliopsoas muscles on 800 m swimming performance in master swimmers.

Scientific reports·2026

Same journal

ISNR-PQC: isometry noise resilience post quantum cryptography primitive.

Scientific reports·2026

Same journal

Identification of high-yielding and stable genotypes of barley in the cold climate of Iran using AMMI and GGE biplot models.

Scientific reports·2026

Same journal

Bayesian negative binomial modelling of spatial and temporal patterns of road traffic deaths in Ghana.

Scientific reports·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 9, 2026

Plasmid Stability Analysis with Open-Source Droplet Microfluidics

Plasmid Stability Analysis with Open-Source Droplet Microfluidics

Published on: December 27, 2024

High-throughput bacterial aggregation analysis in droplets.

Merili Saar-Abroi¹, Karoliine Lindpere¹, Dániel Kácsor¹

¹Department of Chemistry and Biotechnology, Tallinn University of Technology, Tallinn, Estonia.

Scientific Reports

|December 2, 2025

Summary

This summary is machine-generated.

We developed a cost-effective microfluidic droplet method with CellProfiler™ for bacterial aggregation analysis. This standardized approach achieves over 90% accuracy, enabling consistent data for diverse applications.

More Related Videos

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

Published on: May 21, 2020

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

Related Experiment Videos

Last Updated: Jan 9, 2026

Plasmid Stability Analysis with Open-Source Droplet Microfluidics

Plasmid Stability Analysis with Open-Source Droplet Microfluidics

Published on: December 27, 2024

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

Published on: May 21, 2020

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

Area of Science:

Microbiology
Biotechnology
Bioanalytical Chemistry

Background:

Microfluidic droplet platforms offer controlled environments for studying bacterial aggregation.
Current methods lack standardization, leading to inconsistent and incomparable data.
Image analysis is a common technique but often relies on custom, non-reproducible workflows.

Purpose of the Study:

To develop a robust, cost-effective, and standardized method for assessing bacterial aggregation using microfluidic droplets and texture-based image analysis.
To validate the accuracy and precision of the developed method against expert manual evaluation.
To investigate the impact of environmental factors like antibiotics, microplastics, and metals on bacterial aggregation.

Main Methods:

Utilized microfluidic droplet generation (mono- and polydisperse).
Employed texture-based image analysis using the open-source software CellProfiler™.
Compared automated analysis results with manual evaluation by a panel of experts.

Main Results:

The developed method achieved over 90% accuracy and more than 80% precision in bacterial aggregation assessment.
Suboptimal antibiotic concentrations were found to increase bacterial aggregation.
Exposure to microplastic beads and metals reduced bacterial aggregation.

Conclusions:

The developed CellProfiler™-based microfluidic droplet pipeline provides a standardized, accurate, and broadly applicable tool for bacterial aggregation studies.
This method overcomes limitations of custom workflows, enabling consistent and comparable data generation.
The findings highlight the influence of environmental factors on bacterial aggregation dynamics.